杂种卡特兰花粉萌发和花粉贮藏性研究

Germination and Preservation of Rhyncholaeliocattleya Pollen

摘要:

以杂种卡特兰品种‘绿世界’(Rhyncholaeliocattleya Sung Ya Green ‘Green World’)的新鲜花粉为试材,采用正交试验比较了蔗糖、H₃BO₃、CaCl₂对花粉萌发的影响。结果表明, 适宜的花粉培养基为:蔗糖100 g L^-1+H₃BO₃ 40 mg L^-1+CaCl₂ 150 mg L^-1 。 不同贮藏方法对杂种卡特兰花粉萌发率的影响较大,4℃低温湿润贮藏30 d后,花粉萌发率仅为3.5%, 而4℃低温干燥贮藏、-20℃冷冻湿润和干燥贮藏720 d后,花粉仍然具有较高的活力,花粉萌发率均达32.5%以上,且对授粉结实率没有明显影响。杂种卡特兰的授粉最佳时期为开花后0~8 d,花粉块在整个花期均保持较高的活性。

Abstract:

The effects of sucrose, boron and calcium on the germination of Rhyncholaeliocattleya Sung Ya Green ‘Green World’ pollen were studied by orthogonal design experiment. The results showed that the optimum culture medium for pollen germination was 100 g L^-1 sucrose+40 mg L^-1 H₃BO₃ +150 mg L^-1 CaCl₂. Different storage methods had different effects on pollen viability of ‘Green World’. The pollen germination rate was only 3.5% humid stored under 4℃ for 30 days. However, after dry stored in 4℃, freeze-wetting and freeze-drying stored in-20℃ for 720 days, respectively, the pollen germination rate still maintained at high level, reaching up to above 32.5%, and there was not effect on seed setting. The best time for pollination was 0-8 days after flowering and the pollinium had high vitality during whole florescence.