双色真藓的组织培养和发育研究

Tissue Culture and Development of the Moss Bryum dichotomum (Hedw.) in vitro

摘要:

对双色真藓(Bryum dichotomum Hedw.)的孢子发育过程及愈伤组织的诱导和培养进行了研究。结果表明,双色真藓孢子萌发和原丝体发育属于典型的真藓型。将双色真藓原丝体接种在含有2.0 mg L^-1的硅酸钠和3.0 mg L^-1 6-BA的MS固体培养基上,可诱导双色真藓原丝体分化为愈伤组织。愈伤组织在含有2.0 mg L^-1的硅酸钠、1.0 mg L^-12,4-D和1.0 mg L^-1 6-BA的MS固体培养基上可以长期继代培养。而愈伤组织在含有2.0 mg L^-1的硅酸钠、1.0 mg L^-1 2,4-D和1.0 mg L^-1 6-BA的MS液体培养基中可以悬浮培养,且生长迅速,培养28 d达到接种鲜重的9.25倍。

Abstract:

The spore development and callus induction of Bryum dichotomum Hedw. in vitro culture were studied. The results showed that the spore germination and protonema development of B. dichotomum Hedw. were Bryum-type. The calli were successfully induced from protonema cultured on solid MS medium supplemented with 3.0 mg L^-1 6-BA and 2.0 mg L^-1 sodium silicate. The calli could be subcultured at long term on solid MS medium supplemented with 2.0 mg L^-1 sodium silicate, 1.0 mg L^-1 2,4-D and 1.0 mg L^-1 6-BA. The fresh weight of calli cultured for 28 days was about 9.25 times to original weight under suspension culture, when incubated in liquid MS medium supplemented with 2.0 mg L^-1 sodium silicate, 1.0 mg L^-1 2,4-D and 1.0 mg L^-1 6-BA.