麻疯树鲨烯合酶基因SQS的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Squalene Synthase Gene (SQS) from Jatropha curcas L.

摘要:

以麻疯树(Jatropha curcas L.)总RNA为模板,根据已报道的鲨烯合酶基因序列设计简并引物,用RACE方法克隆得到麻疯树鲨烯合酶基因全长cDNA,命名为JcSQS。JcSQS全长1609 bp,包含1个1242 bp的开放阅读框,预测麻疯树鲨烯合酶基因编码的蛋白含有413个氨基酸。JcSQS具有鲨烯合酶类的保守结构域,JcSQS 蛋白与蓖麻、柿、木榄等植物中SQS基因编码的氨基酸序列具有高度同源性。这为研究麻疯树萜烯类物质的生物合成和调控机制奠定了基础。

Abstract:

Using the total RNA extracted from Jatropha curcas L. leaves as the template, the degenerate primers were designed according to the reported sequence of squalene synthases gene in other plants, the full-length cDNA of squalene synthase gene in Jatropha curcas L. was cloned using RACE method, which was named JcSQS with 1609 bp in length including a 1242 bp-ORF, and JcSQS was predicted to encode the protein with 413 amino acids. The cloned JcSQS gene contained the typical conserved sequences and domains of SQS genes. The JcSQS protein had high homology with the amino acid sequences encoded by SQS gene in Ricinus communis, Diospyros kaki and Bruguiera gymnorhiza, so on. It laid the foundation for studying the biosynthesis and regulation of terpenoids and steroids in J. curcas.