利用cDNA-AFLP技术分析龙眼子叶胚基因表达差异

Differential Expression Analysis of Longan Cotyledon Embryo by cDNA－AFLP

摘要:

分别提取‘红核子’龙眼(Dimdcarpus longan Lour.)谢花后35 d (早期)和50 d(晚期)子叶胚RNA，建立cDNA-AFLP分析体系，获得指纹图谱。对41个差异片段回收克隆和序列分析，表明其中21个与已知功能基因具有高度同源性，这些基因的功能主要涉及侧生器官的离轴极性、糖酵解、能量代谢、离子转运、细胞壁伸长、细胞周期调控、RNA转录、RNA翻译和调控、蛋白磷酸化调控和降解、信号转导等；其余片段与已知基因的同源性较低或没有。

Abstract:

The finger map of longan (Dimdcarpus longan Lour. ‘Honghezi’) cotyledon embryo RNA was established by cDNA-AFLP, which extracted from thirty-five-day (early) and fifty-day (late) cotyledon embryos after anthesis, respectively. There were 41 differential fragments cloned and sequence analysed, in which 21 differential expression fragments had high homology with some known genes, the function mainly involved in the abaxial polarity of lateral organ, glycolysis, energy metabolism, ion transport, cell wall elongation, cell cycle regulation, RNA transcription, RNA translation and regulation, protein phosphorylation regulation and protein degradation, signal transduction and so on. While the others had low or no homology with known genes.