谭清苏铁性别相关的RAPD标记研究

Identification of a Sex-associated RAPD Marker in Cycas tanqingii D.Y.Wang
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    摘要:

    以谭清苏铁(Cycas tanqingii D. Y. Wang)雌雄植株半年生羽叶为材料,用优化的CTAB法分别提取其全基因组DNA,进行RAPD单因子梯度实验和正交实验以优化扩增条件。应用160个RAPD随机引物检测基因组DNA,雌雄植株均扩增出1 450多条带,其中引物S0465扩增出与谭清苏铁雌株高度相关的RAPD标记(TQS465-500),其大小约为500 bp,该标记与雄株没有关联。

    Abstract:

    The total genomic DNA of the male and female cycads (Cycas tanqingii D.Y. Wang) was extracted from the leaflets of half a year old using an optimized CTAB protocol. The RAPD-PCR amplification conditions were optimized by monofactor and multifactor orthogonal experiments. A total of 160 primers were used for RAPD-PCR amplification and more than 1 450 fragments were obtained from the male or the female plants. A fragment of about 500 bp only linked to the female was generated steadily by the S0465 (CCCCGGTAAC) primer, which can be used in sex determination of C. tanqingii.

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景建洲, 李东亮, 金红, 张勇, 曹黎明.谭清苏铁性别相关的RAPD标记研究[J].热带亚热带植物学报,2007,15(3):198~202

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