珍稀药用和观赏植物地涌金莲的组织培养和快速繁殖(英文)

Tissue Culture and Rapid Propagation of Musella lasiocarpa, a Rare Chinese Medicinal and Ornamental Herb

摘要:

以地涌金莲吸芽为外植体建立了有效的快繁体系。外植体经灭菌处理后在MS+6-BA 4.0 mg L^-1+NAA 0.2 mg L^-1+维生素C 150 mg L^-1+10%椰子乳+3%蔗糖培养基上能进行不定芽的诱导和增殖，培养60 d后每个芽平均能产生4.10个不定芽。第6代增殖后，丛生芽的增殖系数可达4.23。生根培养基以1/2MS+NAA 1.0 mg L^-1+AC 50 mg L^-1的效果较好。以沙：泥炭土:沙=1:1：1为基质移栽试管苗,成活率达到93.5%以上。通过12个月的组织培养已生产1 0000多株试管苗。

Abstract:

An efficient and rapid micropropagation system by using auxiliary buds as explants has been established for Musella lasiocarpa. Murashige and Skoog (MS) medium supplemented with 4.0 mg L^-1 6-benzylaminopurine, 0.2 mg L^-1 1-naphthaleneacetic acid, 150 mg L^-1 vitamin C, 10% coconut milk and 3% sucrose was suitable for shoot induction and proliferation from divided buds. Each explant produced an average of 4.10 shoots after 60-day culture. After the sixth subculture, the shoot proliferation rate of plantlets was 4.23-fold. The half-strength MS medium supplemented with 1.0 mg L^-1 indole-3-butyric acid and 150 mg L^-1 activated charcoal was suitable for rooting. When micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand, sieved peat and perlite (1:1:1; v/v) in a greenhouse, 93.5% of the plantlets survived. About 10 000 plantlets were produced successfully for field transfer after 12 months of culture initiation. This production system is useful for ex situ conservation and large-scale multiplication of M. lasiocarpa.