莱菔子脂肪酸成分的GC-MS分析

Analysis of Fatty Acids in Raphanus Seeds by GC-MS

摘要:

采用索氏提取法提取莱菔（Raphanus sativus）种子中的脂肪酸成分，进行甲酯化处理后用气相色谱-质谱联用技术分离和鉴定脂肪酸成分的组成和含量。结果表明，从莱菔子中共分离鉴定出12种脂肪酸成分，占总量的99.32%，主要包括芥酸（32.47%）、油酸（29.07%）、亚油酸（9.45%）、亚麻酸（8.41%）、棕榈酸（4.31%）和硬脂酸（2.08%）等脂肪酸，其中饱和脂肪酸占9.25%，不饱和脂肪酸高达90.07%。

Abstract:

The fatty acids from the seeds of Raphanus sativus were extracted with petroleum ether by Soxlet extraction. The extracted fatty acids were esterified and analyzed by gas chromatography-mass spectrometry. Twelve fatty acids were detected and identified, amounting to 99.32% of the total fatty acids, mainly including 13-docosenoic acid （32.47%）, 9-octadecenoic acid （29.07%）, 9,12-octadecadienoic acid （Z,Z） （9.45%）, 9,12, 15-oetadecatrienoic acid （8.41%）, hexadecanoic acid （4.31%）, and octadecanoic acid （2.08%）. The saturated fatty acids and unsaturated fatty acids were 9.25% and 90.07% in amount, respectively.