不同亲和性水稻材料的同工酶遗传多样性分析

Genetic Diversity among Different Compatible Varieties in Rice （Oryza sativa L.） Using Isozyme Markers

摘要:

采用同工酶标记对亲籼、亲粳、广亲和及非亲和4组不同亲和性水稻材料的遗传变化和遗传结构进行分析.10种同工酶在95个材料中共检测到34个同工酶位点和55个等位基因,其中多态位点15个,多态位点百分率为44.12%.各组的平均期望杂合度在0.354到0.456之间,在物种水平上为0.454.AMOVA分析表明,80.21%的遗传变化分布于组内,19.79%的遗传变化分布于组间.各组间的遗传距离变化范围从0.1129到0.3673,基因流变化范围从1.0242到2.5451.根据遗传距离进行的UPGMA聚类分析,将广亲和与亲粳水稻聚为一类,亲籼与非亲和水稻聚为一类.

Abstract:

A total of 95 rice varieties were collected including four compatibility groups of indica compatible varieties （ICV）, japonica compatible varieties （JCV）, wide compatible varieties （WCV） and incompatible varieties （NCV）. Genetic variation and genetic structure among the four groups were evaluated by using isozyme markers. Ten isozymes gave rise to 55 alleles in 34 loci of which 15 （P = 44.12%） were polymorphic. The average expected heterozygosity ranged from 0.354 to 0.456 at the group level, and 0.454 at the species level. AMOVA analysis showed that 80.21% of genetic variance distributed within groups, and only 19.79% among groups. The variation of genetic distance between groups ranged from 0.1129 to 0.3673, and the gene flow ranged from 1.0242 to 2.5451. UPGMA cluster analysis based on Nei＇s genetic distance could divide the four groups into two clusters:( Ⅰ ) wcv and JCV, ( Ⅱ ) ICV and NCV.