两步外植体法高频再生麝香百合

A Two-step Method for the Efficient Micropropagation of Lilium longiflorum

摘要:

采用两步外植体法，即以百合鳞片叶为初始外植体，以从初始外植体上长出的芽为次级外植体，成功建立了麝香百合的高频离体再生系统。对不同的BA浓度及次级外植体的不同部位对再生效果的影响，以及组培苗移栽前低温处理的影响进行了研究。结果表明：不同部位的次级外植体中，以短缩苹切片出芽快、整齐、芽数多且粗壮；以MS附加1.0 mg L^-1 BA和0.1 mg L^-1NAA的培养基最适于麝香百合的分化。一个中等大小已脱春化的鳞茎通过两步外植体法能扩繁出54000株左右的新植株，从鳞片叶开始至开花仪需8个月，而且4℃低温处理对开花期的影响不大。

Abstract:

Bulb scale segments of Lilium longiflorum were used as original explants and shoot sections excised from shoot clusters arosed from the first explants as the secondary explants. The effects of different concentrations of BA （benzyladenine） and different parts of plant segments on shoot formation were studied. After roots formed, plantlets were treated at 4℃ for 0, 7 and 30 days. The results indicated that MS medium supplemented with 1.0 mg L^-1 BA and 0.1 mg L^-1 NAA was optimum for shoot formation and proliferation by using short basal stem segment as the second explant. Moreover, cold treatment had little effect on flowering stage. About 54 000 new plantlets could be obtained from one bulb. It took only 8 months from bulb scale to flowering.