太子参细胞悬浮培养及其皂苷含量分析

Cell Suspension Culture of Pseudostellaria heterophylla and Its Saponin Content Analysis

摘要:

以太子参的幼叶为外植体,诱导培养获得太子参愈伤组织,并通过细胞悬浮培养获取皂苷。结果表明：用MS＋BA 0.2 mg L^-1＋2,4-D 1.0 mg L^-1＋KT 1.0 mgL^-1液体培养基可获得大量繁殖速度快、生长均匀一致的悬浮细胞。由细胞悬浮培养获得的太子参皂苷的HPLC色谱峰值与常规种植及组培苗的相同,但纯度较好。细胞悬浮培养约30 d时,每克干重细胞的培养液内可提取总皂苷量为2.13-2.92 mg,略低于大田常规种植所收获的每克干重太子参块根内的总皂苷含量（3.6-4.3 mg）,与组培苗收获的太子参块根内的总皂苷含量相近。

Abstract:

Callus was induced from the young leaves of Pseudostellaria heterophyll （Miq.） Pax and saponin was obtained by way of cell suspension culture. Plenty of incompact and rapid growing suspension cells were obtained from cultures grown in liquid medium of MS supplemented with 0.2 mg L^-1 BA, 1.0 mg L^-1 2,4-D and 1.0 mg L^-1 KT. The HPLC chromatograms of saponin from suspension cell culture to that from conventionally field-grown or tissue culture seedlings. After about 30 days＇cell suspension culture, the total content of saponin in cultural fluid reached 2.13-2.92 mg g^-1 DW cells, which was slightly lower than that from the roots of conventionally field-grown seedlings, and was close to that from the roots of tissue culture plantlets.