乙醇溶液对木瓜蛋白酶催化活性的影响

Effect of Ethanol on Catalytic Activity of Papain

摘要:

研究了乙醇溶液对木瓜蛋白酶水解酪蛋白的催化活性及构象的影响。结果表明，木瓜蛋白酶在一定浓度乙醇溶液中水解酪蛋白的活性有显著上升。动力学测定表明木瓜蛋白酶在乙醇溶液中米氏常数（Km）下降。差示光谱显示，在乙醇溶液中木瓜蛋白酶的二级结构发生了变化。荧光发射光谱表明，木瓜蛋白酶在乙醇溶液中发射峰位几乎没移动，但发射强度明显增高。

Abstract:

The alterations of papain activity and conformation were studied in condition of pretreatment with ethanol solutions at different concentrations, different pretreatment times, and in condition of various reaction concentrations. The result showed that the papain activity was obviously increased and the Km value was declined when treated with ethanol. Ultraviolet difference spectra showed that the secondary structure ofpapain altered in ethanol solutions. Fluorescence emission spectra showed that the peak of fluorescence emission was not shifted but the fluorescence intensity in papain increased in ethanol solutions.