实验室培养球形棕囊藻溶血毒素的提取、分离及其生成特征

Extraction of Haemolytic Substances from Alga Phaeocystis globosa

摘要:

研究从实验室培养的球形棕囊藻(Phaeocystisglobosa)提取溶血毒素的条件, 探讨不同生长期球形棕囊藻溶血毒素的生成特征, 用薄层色谱法对溶血成份进行了初步分析。结果表明, 球形棕囊藻细胞壁的超声波破碎最适条件为:功率600 W, 4℃下处理30 min。对数生长期、平稳期和衰亡期藻细胞适宜处理量分别为每次3 000、2 000、1 000 ml；在球形棕囊藻生长的平稳期和衰亡期具有明显的溶血活性, 对数生长期溶血活性很低, 甚至检测不到。球形棕囊藻溶血毒素至少含有4种糖脂类化合物。

Abstract:

The extraction of haemolytic toxin from Phaeocystis globosa was studied under laboratorial condition at various algal growth phases. Optimal conditions for breaking algal cell wall by ultrasonic was 600 W of power treated for 30 minutes at 4℃, the maximum volume of cultures being 3 000, 2 000 and 1 000 ml at logarithmic, stationary and senescent phases, respectively. High haemolytic activity was observed in stationary and senescent phases, but low or even nil in logarithmic phase. At least four haemolytic substances were found in this alga.