阳桃的组织培养与快速繁殖

Rapid in vitro Propagation of Averrhoa carambola

摘要:

以阳桃茎段为材料进行组培快繁。结果表明阳桃离体培养的细胞分裂素以ZT的效果最好,其次是BA,KT最差;植物生长调节物质是NAA最好,其次是IBA、IAA;较适宜的植物生长调节物质配比为BA 0.5 mg L^-1+NAA 0.2 mg L^-1+GA 0.2 mg L^-1,增殖系数高达3.7,而且畸苗率较低(23.3%)。生根培养基以1/2MS+IBA 0.2 mg L^-1+IAA 0.1 mg L^-1效果好,生根率达87.1%,蔗糖浓度以2.0%-3.0%为宜。

Abstract:

Nodal regments of tropical fruit Averrhoa carambola (Oxalidaceae) were used as explants to investigate optimum conditions for rapid propagation. Among cytokinins (1.0 mg L^-1), zeatin (1.0 mg L^-1) was best for culture, and kinetin (1.0 mg L^-1) the worst. MS medium supplemented with 0.5 mg L^-1 BA, 0.2 mg L^-1 NAA and 0.2 mg L^-1 GA was optimal for the plantlet growth, the proliferation coefficient being as high as 3.7, and the rate of abnormal plantlets was only 23.3%. As for rooting, half-strengh MS medium containing 0.2 mg L^-1 IBA，0.1 mg L^-1 and 2％-3％ sucrose was the best．The rooting rate was 87.1％ ．