不同温湿条件下贮藏的3种禾本科植物花粉的一些生理变化

Changes in Proteinase Activity, Proteins and Amino Acids Contents in Three Species of Grass Pollens Stored under Different Temperature and Humidity Conditions

摘要:

测定了水稻、玉米和狼尾草花粉在低温(4℃)低湿(RH45%),低温(4℃)高湿(RH90%),高温(35℃)低湿(RH45%)和高温(35℃)高湿(RH90%)4种贮藏条件下的蛋白酶活性,蛋白质含量,游离氨基酸含量的变化,结果表明:在低温高湿条件下,蛋白质降解和游离氨基酸含量上升慢；在高温条件下蛋白质降解快,游离氨基酸含量迅速上升。3种植物花粉中,水稻花粉蛋白酶活性强,在贮藏过程中蛋白质降解速率和游离氨基酸含量上升快；狼尾草花粉蛋白酶活性低,在贮藏过程中蛋白质降解速率和游离氨基酸含量上升慢；玉米花粉的蛋白酶活性、蛋白质降解速率和游离氨基酸含量上升速率居两者之间。

Abstract:

The pollens of rice (Oryza sativa), maize (Zea mays) and Pennisetum alopecuroides were stored under low temperature (4℃) how humidity (RH 45%) , low temperature (4℃) high humidity (RH 90%), high temperature (35℃) how humidity (RH 45%) and high temperature (35℃) /high humidity (RH 90%) to determine the changes in proteinase activity, contents of proteins and free amino acids in the pollens. The results showed that the degradation of protein and the increase of the content of free amino acids were slow under low temperature and high humidity, but it was rapid under high temperature. Among the pollens of the three species, proteinase activity in rice pollen was high, and the degradation of protein contents and the increase of free amino acids were rapid. However, proteinase activity in pollen of Pennisetum alopecuroides was low, and the changes in protein content and free amino acids were slow. These changes in maize were intermediate between the above two species.