银杏愈伤组织培养及其黄酮类化合物的测定(简报)

CALLUS CULTURE OF GINKGO AND THE CONTENT OF FLAVONOID COMPOUNDS IN THE CALLI

摘要:

采用银杏胚、胚乳及3个月苗龄的苗叶为外植体，在附加不同激素的培养基上诱导出愈伤组织，从愈伤组织中提取黄酮类化合物并测定其含量。结果表明，由胚诱导的愈伤组织中黄酮类化合物含量最高。

Abstract:

The embryos, endosperm and young leaves of 3-month-seedling of Ginkgo biloba L. were used as explants for callus induction experiment. Calli induced from embryos on MS medium supplemented with 3 mg L^-1 NAA and 5 mg L^-1 KT gave highest growth rate (100%), whereas calli induced from endosperms on MS with 3 mg L^-1 NAA and 2 mg L^-1 KT gave 42.3% growth rate. MS with 1 mg L^-1 NAA and 3 mg L^-1 BA was best for callus induction from leaves. The content of flavonoid compounds was highest in calli from embryos, and lowest in calli from embryos, and lowest in calli from endosperms.