若干理化因子对日本肾蕨绿色球状体分化及生长的影响

THE EFFECTS OF PHYSICAL AND CHEMICAL FACTORS ON DIFFERENTIATION AND GROWTH OF GREEN GLOBULAR BODIES OF NEPHROLEPIS CORDIFOLIA

摘要:

通过对日本肾蕨（Nephrolepis cordifolia)绿色球状体（GGB）的培养实验，研究了不同物理、化学因子对其分化及生长的影响。结果表明，辐照度100 μmol m^-2s^-1对GGB的分化及生长均有明显促进作用。红外照射对GGB的分化比白光和蓝光的好。此外，GGB的分化以MS＋6-BA 1 mg L^-1+NaH₂PO₄ 200 mg L^-1为好。

Abstract:

Green globular bodies (GGB) first obtained from runner tips of Nephrolepis cordifolia Presl were cultured on MS medium supplemented with 4 mg L^-1 BA and 0.1 mg L^-1 IAA at 23 ℃ under continuous fluorescent light at 40 μmol m^-2s^-1 for 30 days. These GGB were then subcultured on the same medium every month. The differentiation and the growth of GGB subcultured on varies media or with different growth regulators, and various light quality and light intensity were examined and calculated after 70 days. The results showed that stimu1ating effects on the differentiation and growth were observed at irradiance of lO0 μmol m^-2s^-1．Red light was better for differentiation of GGB than blue or white light．In addition，MS medium supplemented with 1 mg L^-1 6-BA and 200 mg L^-1 NaH₂PO₄ was best for differentiation of GGB．