芦荟凝集素的分离、纯化和部分性质的研究

THE ISOLATION AND PURIFICATION OF LECTIN FROM ALOE VERA L. VAR. CHINENSIS (HAW.) BERGER AND ITS CHARACTERISTICS

摘要:

新鲜芦荟叶(Aloe vera L. var.chinensis(Haw.)Berger)于室温用低浓度NaCl溶液提取、离心和透析后,经N-乙酰氨基葡萄糖-Sepharose 4B亲和层析,分离纯化出芦荟凝集素（ACL）。用Sephadex G-100测表观分子量为35 kD,SDS-PAGE出现两条色带:染色深的宽带和较浅的窄带。亚基分子量分别为15 kD和20 kD。能专一性凝集兔血细胞和人血红细胞的A型和O型,不凝集B型和AB型。D-甘露糖和N-乙酰氨基葡萄糖能抑制凝血活性。糖蛋白内中性糖含量是4.8%。

Abstract:

Lectin was extracted from leaves of Aloe vera L. var. chinensis (Haw.) Berger in a dilute NaCI solution. After centrifugation, the collected supernatant was dialysed. Pure lectin of Aloe (ACL) was isolated by N-acetylglucosandne-Sepharose 4B affinity chromatography and purified by Sephadex G-100 gel filtration chromatography. The apparent molecular weight of ACL was 35 kD. It consisted of two subunits, 15 kD and 20 kD. ACL could agglutinate rabbit erythrocytes and the human erythrocytes with groups A and O specifically, except those with groups B and AB．The activity of ACL was inhibited by D-mannose and N-acetylglucosamine．Neutral sugar content in ACL was 4.8％.