利用RAPD标记研究荔枝品种的新缘关系

IDENTIFYING LITCHI CULTIVARS AND EVALUATING THEIR GENETIC RELATIONSHIPS BY RAPD MARKERS

摘要:

采用改进的CTAB方法提取34个荔枝品种的基因组DNA为模板，从50个10碱基的随机引物中获得了37个多态性RAPD标记，聚类分析结果表明这34个荔枝品种可以分成4组，第一组有“状元红”、“元红”、“桂林”、“宋家香”、“台湾荔”、“下番枝”、“东刘一号”、“妃子笑”、“白糖罂”、“香丸”、“绿沙”；第二组有“无栗子”、“水东”、“乌叶”、“蔡家肉丸”、“青壳糯米糍”、“糯米糍”；第三组有“桂味”。

Abstract:

In this paper, we used modified CTAB method to extract genomic DNA from litchi for amplifying RAPD markers. We got 37 polymorphic RAPDs out of 259 total RAPDs from 34 litchi cultivars with 50 random primers. The results showed that these 34 cultivars could be divided into 4 groups. Group I included "Zhuangyuanhong", "Yuanhong", "Guilin", "Songjiaxiang", "Taiwanli", "Xiafanzhi", "Dongliu No. l", "Feizixiao", "Baitangying", "Xiangwan", "Lusha". Group II comprised "Wulizi", "Shuidong", "Wuye", "Caijiawanzi","Qingkenuomici"," Nuomici",Group III included "Guiwei", "Guangming"," Bailizhi","Chenzi" , "Lanzhu", "Jidi", "Taslniu". Group Ⅳ consisted of "Huitoujiang","Putaoben", "HuaizhV", "Nanhaili", "Dazao", "Zaohong"，"Huoshaoli", "Wuyejiu"，"Midingxlang", "Luhebao". The results of cluster suggested that the polymorphism of litchi germplasm was narrower than expected.