利用同工酶和SDS-PAGE技术对一些兰属(Cymbidium)品种的分析(简报)

GERMPLASM ANALYSIS IN SOME CYMBIDIUM CULTIVARS USING ISOENZYME AND SDS-PAGE

摘要:

利用同工酶和SDS-PAGE技术对兰属（Cymbidium）5个种和2个变种的11个品种进行了分类研究，酯酶和细胞色素氧化酶的同工酶共出现24条带，其中23条具有多态性；SDS-PAGE有30条带，其中25条带为多态性。根据同工酶和SDS-蛋白质标记进行聚类分析，生化水平与传统的形态学分类结果基本一致，但某些兰属品种的分类地位与传统的分类有一些差异。

Abstract:

Isoenzyme and SDS-PAGE techniques were used to analyze 11 cultivars of genusCymbidium. Among 24 bands of esterase and cytochrome oxidase, 23 were polymorphic,and 25 of 30 bands produced in SDS-PAGE were polymorphic. Based on the clusteranalysis of these markers, biochemical results were consistent with those of traditionaltaxonomic study, except that the systematic status for several Cymbidium cultivars weredifferent from the mophological classification.