细胞质雄性不育水稻幼穗和花药的呼吸酶活性研究

ACTIVITIES OF RESPIRATORY ENZYMES AT DIFFERENT DEVELOPMENTAL STAGES OF PANICLES AND ANTHERS IN CMS RICE AND ITS MAINTAINER

摘要:

研究珍汕97A和珍汕97B的雌雄蕊原基形成期、花粉母细胞形成期和花粉母细胞减数分裂期的幼穗及单核期、二核期和三核期的花药中呼吸代谢三羧酸循环（TCA）的苹果酸脱氢酶（MDH）和异柠檬酸脱氢酶（IDH）及戊糖途径（PPP）的磷酸葡萄糖脱氢酶（G6PDH）、磷酸葡萄糖酸脱氢酶（6PGDH）和5一磷酸核糖异构酶（RSPI）的活性。结果表明：可育花药的5种酶活性皆高于同期不育花药；而幼穗中，TCA途径中的MDH和IDH在不育系与保持系之间无差异，PPP途径的G6PDH和6PGDH及R5PI则保持系高于不育系。这说明不育系中PPP发生的变化早于TCA途径，PPP途径的改变可能与小孢子败育有着更为直接的关系。

Abstract:

The activities of respiratory enzymes in cytoplasmic male sterile (CMS) rice Zhenshan 97A and its maintainer Zhenshan 97B were studied. The results showed that the activities of malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), glucose-6phosphate dehydrogenase (G6PDH), 6-phospho-gluconate dehydrogenase (6PGDH) and 5-Phosphoribose isomerase (RSPI) in sterile anthers were all lower than those in fertile ones.But in panicles only the activities of G6PDH, 6PGDH and RSPI in sterile line were lower than those in fertile line. The results indicated that the cytoplasmic sterility of rice was associated with the obvious decrease of tricarboxylic acid cycle(TCA) and pentose phosphate pathway (PPP) activities, and the change in PPP operation was shown to be earlier than that in TCA.