10升气升环流式生物反应器培养紫草细胞

CELL SUSPENSION CULTURE OF LITHOSPERMUM ERYTHRORHIZON IN A 10-LITRE AIRLIFT LOOP BIOREACTOR

摘要:

本文采用自行设计研制的10升气升环流式生物反应器培养紫草细胞，培养周期34d．前14d为细胞生长培养，细胞生长呈正常的S型曲线，细胞增长到原细胞接人量的4倍．后20d为紫草色素生产培养，细胞增长到32倍。整个周期每升培养液可生产紫草色素0．6g，在反应器中，培养液pH值的变化与细胞生长呈正相关，与紫草色素的形成呈负相关，pH值变化规律可用于监测紫草细胞在生物反应器的生长和色素形成．

Abstract:

A 10-litre airlift loop bioreactor was tested for cell suspension culture of Lithospermum eqthrorhizon, the procedure of which was divided in two steps. In the first 14 days, the cells were cultured with modified B5 medium, the cell increment being 4 times as compared with the 2.5 g L-1 of inoculating cells (dwt). In latter 20 days, the cells were cul- tured with modified M9 medium, in which the amount of cells increased again 3.2 times as compared with that at the end of culture in this medium, and 0.6 g of shikonin derivatives per litre of medium can be obtained in a culture cycle. The pH in modified B5growth medium rose with cell increasing in bioreactor. The change in pH of B5 medium had positive relation to the amount of cell growth. On the contrary, pH in M9medium declined with the timeroourse of the formation of shikonin derivatives, which had a negative relation between pH and shikonin formation. It is suggested that the property of cell growth and the formationof shikonin derivatives could be detected by the pH level in the medium of cell suspension cultures in bioreactor.