湖北光周期敏感核不育水稻农垦58S幼穗和花药发育期的蛋白酶活性变化

CHANGES IN ACTIVITIES OF PROTEOLYTIC ENZYMES IN HUBEI PHOTOPERIOD SENSITIVE GENIC MALESTERILE RICE AT THE STAGES OF PANICLE AND POLLEN DEVELOPMENT

摘要:

湖北光周期敏感核不育水稻农垦585的可育与不育株在一次枝梗期的幼穗中可溶性蛋白含量相近。雌雄蕊形成期和花粉母细胞分化期的可有株可溶性蛋白较不育株的分别高21％和12．3％；可有株在花粉二核和三核期亦有较高含量。不育株在雌雄蕊形成期后，酪蛋白酶活性迅速增高，而可有株至二核期才增高。不育株除在二次枝梗期的内肽酶活性略低外，其余阶段酶的活性较高。育性转变时内肽酶类型亦发生变化。可有株有较高的氨肽酶活性．

Abstract:

The content of soluble protein in panicle of Hubei photoperiod sensitive genic male-sterile rice, Nongken 58S, was neariy the same in both fertile and sterile plants at the stage of first panicle branch differentiation. Soluble protein contents in fertile plant at the stage of pistil and stamen formation and the stage of pollen mother cell differentiation were higher than those in the sterile one by 21% and 12.3%, respectively. Higher soluble protein content occurred at binucleate and trinucleate stages for the fertile plant. The activity of azocasein protease increased rapidly at the stage of pistil and stamen formation in male-sterile plant, whereas this process was not present in fertile plant until binucleate stage. Male-sterile plant had lower endopeptidase activity at the stage of second panicle branch differentiation, and higher activity of this enzyme appeared at other stages as compared with the fertile plant. The endopeptidase patterns changed with the fertility. Higher aminopeptidase was observed in the fertile plant of rice.