Abstract:The spatial distribution patterns of community biomass and number of individual, and the distribution pattern of dominant populations responsed to road under different sampling scales were studied in pine and broad-leaved mixed forest along the road in Dinghushan Nature Reserve, Guangdong Province. Sample plots were arranged along transects vertical or parallel to road. The results showed that there were no significant difference in community biomass among the sampling scales along parallel direction of road (P>0.05), while significant increase in number of individuals among sampling scale were detected (P<0.05). On the sampling scale of 20 m, the road effects on different dominant populations were demonstrated in their spatial distribution patterns. There were significant increases in the biomass of Schima superba population, the number of individuals and biomass of Litsea rotundifolia population (P<0.05) along the parallel direction to road, respectively, while those of other populations had no significant difference.