扁蓿豆体细胞胚的诱导和植株再生

INDUCTION OF SOMATIC EMBRYOS AND PLANTREGENERATION OF MELISSITUS RUTHENICA L.

摘要:

扁蓿豆实生苗的根、下胚轴、子叶、叶片和叶柄外植体，在含２，４—Ｄ２—０．２５ｍｇＬ－１与ＫＴ０．２５－２ｍｇＬ－１及２，４—Ｄ０．５ｍｇＬ－１与ＺＴ０．５ｍｇＬ－１或ＢＡＰ０．５ｍｇＬ－１与ＮＡＡ０．０５ｍｇＬ－１的ＭＳ琼脂培养基上均可产生愈伤组织．愈伤组织在含２，４—Ｄ０．５—０．１ｍｇＬ－１与ＫＴ０．５—０．１ｍｇＬ－１或ＢＡＰ０．２５＋ＮＡＡ０．０５ｍｇＬ－１的ＭＳ培养基上可诱导分化出体细胞胚．体细胞胚在无激素的培养基上发育成完整植株．用海藻酸钠包襄体细胞胚制成人工种子，其发芽率和植株转换率分别为９５％和５３％．

Abstract:

Explants of root,hypocotyl,cotyledon,leaf and petiole from seedlings of Melissitusruthenica L.could produce callus tissues when they were cultured on MS agar mediumcontaining 2,4-D 2-0.25mg L-l and KT 0.25-2mg L-1,or 2,4-D 0.5mg L-1 and ZT0.5mg L-1,or BAP 0.5mg L-1 and NAA 0.05mg L-1.Callus tissues Could be induced todifferentiate somatic embryos when they were transfered to MS medium containing 2,4-D0.5-0.1ing L-1 and KT 0.5-0.1mg L-1,or BAP 0.25mg L-1 and NAA 0.05mg L-1.Somatic embryos could develop into whole plants on hormone free MS medium.Somaticembryos were encapsulated by 1.5% alginate to prepare artificial seeds,of whichgermination rate and plant conversion rate were 95% and 53%, respectively.