茶(Camellia sinensis Kuntze)离体培养体细胞胚胎发生的组织细胞学研究
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HISTO-CYTOLOGICAL STUDY ON SOMATIC EMBRYOGENESIS FROM THE LEAF OF CAMELLIA SINENSIS KUNTZE
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    摘要:

    以茶树叶片为外植体.以MS培养基附加4mg·L-16-BA,2mg·L-1IAA.3mg·L-1GA3和0.2—0.3%活性碳诱导出愈伤组织和胚状体,进一步形成小植株.切片观察表明.茶叶愈伤组织胚状体的发生,起源于愈伤组织表层及其内部的单个细胞和细胞团,胚状体发育顺序与合子胚大致相似.经球形胚、心形胚、鱼雷胚和子叶胚阶段.但发育过程中常有畸形胚出现.

    Abstract:

    Leaf-derived callus of Camellia sinensis Kuntze was obtained on MS medium supplemented with 6-BA (1 or 4mg·L-1 ) +IAA (2mg·L-1 ) +GA3 (3mg. L-1 ) +activated carbon (0. 2- 0. 3% ). On the same medium, embryogenesis was induced and finally formed plantlets. It was observed from the sections that the embryoids derived either from the cell or the cell mass on the surface of the callus, or from the cell or the cell mass in the deep inner pert of the callus. The developing order of embryoids was approximately the same as that of zygote embryos. They developed into plantletS via the stage of globular embryo, heart-shaped embryo, torpedo-shaped embryo and cotyledon embryo.Many abnormal embryoids appeared in the developing process.

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王毅军,严学成,暨淑仪,魏新姐.茶(Camellia sinensis Kuntze)离体培养体细胞胚胎发生的组织细胞学研究[J].热带亚热带植物学报,1994,2(2):47~51

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