重复DNA顺序pRRD9在稻属中的拷贝数测定

DETERMINATION OF COPY NUMBER OF REPETITIVE DNA SEQUENCE pRRD9 IN GENUS ORYZA

摘要:

本文应用狭缝印渍杂交方法,把水稻基因组总DNA和含水稻中度重复顺序片段的质粒(pRRD9)DNA分别转移到尼龙膜上形成狭缝印渍、然后用³²P标记的 pRRD9插入片段进行杂交、根据各狭缝印渍的放射性强度,测定水稻(Oryza)一些栽培种和野生种基因组中重复DNA顺序的拷贝数,并就拷贝数与水稻进化关系及基因组型的联系进行讨论.

Abstract:

Copy number of cloned repetitive DNA sequence pRRD9 has been determined within the genus Oryza, including 15 cultivated rice (7 belonging to Oryza sativa L. Japonica, 8 belonging to Oryza sativa L. Indica) and 16 wild rice entries (belonging to AA, A~I A~I, A~cu A~cu, BBCC, CC, CCDD, EE and FF genome types respectively). This was done by using quantitative filter hybridization technique named'SLOT-BLOTTING'. The result shows that pRRD9 copy number is markedly variable between cultivated and wild rice entr...