Nd3+对GA3诱导小麦α－淀粉酶活性的促进作用

THE PROMOTION OF Nd3+ ON α-AMYLASE ACTIVITY INDUCED BY GIBBERELLIC ACID

摘要:

1 ppm Nd³⁺能够明显促进GA₃对α-淀粉酶的诱导,使该酶活性提高 70％,并降低半粒小麦电解质外渗.Nd³⁺的主要效应之一在于缩短GA_3作用的滞后期。其增效现象在GA₃诱导α-淀粉酶的线性浓度范围内,均较显著.Nd³⁺本身不能诱导α-淀粉酶,未发现其对离体酶活性与还原糖显色有影响.

Abstract:

lppm Nd³⁺ promoted the α-amylase activity induced by gibberellic acid at concentrations from 10^-9 to 10^-7 mol/L in, embryoless. wheat seed The activity of α-amylase in the seed treated with lppm Nd³⁺ and 10^-8 mol/L GA₃ was 70% more than that just treated with GA₃. Nd³⁺(lppm) did not induce α-amylase activity and did not affect the enzyme activity in vitro, but it shortened the stagnant time of GA₃ action. It abo decreased the electroconductivity of the medium in which the embryoless seeds were cultured.