天女木兰花粉形态特征及其生活力

Pollen Morphology and Viability of Magnolia sieboldi

摘要:

对天女木兰(Magnolia sieboldii)花粉的采集时期、形态以及培养的温度和培养基进行了研究, 并探讨了贮存温度和时间对花粉生活力的影响。结果表明, 扫描电镜观察到的花粉形状和萌发孔与其他已报道的木兰科植物相同, 但大小和花粉外壁的纹理不同。在花瓣刚刚展开的初花期采集的花粉生活力最高。25℃下最适于花粉的萌发; 花粉在含10%蔗糖+0.1%硼酸的培养基上培养14 h的萌发率最高, 0.01%~0.05%的氯化钙对花粉的萌发影响不显著, 0.1%的氯化钙对花粉的萌发有抑制作用。随着贮存时间的延长, 花粉萌发率不断降低。不同贮存温度下花粉萌发率的降低速度依次为-10℃＜4℃＜25℃, -10℃下贮藏5 d的花粉萌发率降低了48.8%, 10 d后萌发率降低为0。

Abstract:

The collection, morphology characters and culture condition of pollens of Magnolia sieboldii were studied, and the effects of storage temperature and time on pollen viability were investigated. The pollen morphological characters and germinal aperture of Magnolia sieboldii under scanning electron microscope are similar to that of the other species in Magnoliaceae, but the size and exine sculpture are different. The pollen collected in the initial bloom stage has the highest germination rate at 25℃. Pollens cultured on medium with 10% sucrose and 0.1% boric acid for 14 hours had the highest germination rate. The 0.01%~0.05% CaCl2 had no significant effect on pollen germination, but 0.1% CaCl2 had negative effect. The viability of pollen of Magnolia sieboldii decreases gradually as increasing of preservation at different storage temperature, and the pollen viability are decreased in order of 25℃>4℃> -10℃. The pollen germination rate reduced from 87.9% to 48.8% when they were stored at -10℃ for 5 days and reduced to 0 for 10 days.