%0 Journal Article %T 三角梅cDOPA 5GT基因的克隆和光照对其表达的影响 %T Cloning of Cyclo-DOPA-5-glucosyltransferase Gene from Bougainvillea glabra and Effect of Illumination on Its Expression %A 刘姗,孙蓉,高静雷 %A LIU,Shan %A SUN,Rong %A GAO,Jing lei %J 热带亚热带植物学报 %J Journal of Tropical and Subtropical Botany %@ 1005-3395 %V 29 %N 1 %D 2021 %P 75-81 %K 三角梅;葡糖基转移酶;基因表达;光照 %K Bougainvillea glabra;Cyclo-DOPA-5-glucosyltransferase;Gene expression;Illumination %X 为了解光照对三角梅(Bougainvillea glabra)葡糖基转移酶基因表达的影响,从其苞片中克隆了环多巴5-糖基转移酶基因(cDOPA 5GT)。结果表明,三角梅cDOPA 5GT基因的cDNA全长为1 446 bp,编码482个氨基酸,生物信息学分析表明cDOPA 5GT蛋白的等电点(PI)为5.77,具有糖基转移酶的特征基序,为酸性亲水跨膜蛋白,不含信号肽,二级结构以α-螺旋和无规则卷曲为主,氨基酸序列保守性较差。qRT-PCR分析表明,遮光处理cDOPA 5GT基因表达量和植株的甜菜红素含量均显著下降。这表明三角梅的色素合成受糖基转移酶基因调控,并与光照呈正相关关系。 %X In order to understand the effect of illumination on the expression of glucosyltransferase gene in Bougainvillea glabra, the cyclo-DOPA-5-glucosyltransferase gene (cDOPA 5GT) was cloned from bracts. The results showed that the full length cDNA of cDOPA 5GT was 1 446 bp, encoding 482 amino acids. The bioin-formatics analysis showed that isoelectric point of cDOPA 5GT was 5.77, showing an acidic and hydrophilic transmembrane protein without signal peptide. The cDOPA 5GT protein had characteristic motifs of glucosyl-transferase, and the secondary structure was dominant of α-helices and random coils, and the amino acid sequence was less conserved. The results of qRT-PCR and spectrophotometry showed that the expression of cDOPA 5GT gene and betacyanin accumulation in plants decreased significantly under shading by qRT-PCR and spectro-photometry. Therefore, it was suggested that the synthesis of betacyanin was regulated by glucosyltransferase gene, and positively correlated with light time. %R 10.11926/jtsb.4233 %U http://jtsb.ijournals.cn/jtsb_cn/home %1 JIS Version 3.0.0