珍稀濒危植物长梗木莲萃取物的抗肿瘤、抗炎及抗氧化活性研究

Study on the Anti-tumor, Anti-inflammatory and Antioxidant Activities of Extracts from the Rare and Endangered Plant Manglietia longipedunculata

摘要:

长梗木莲(Manglietia longipedunculata)为木兰科(Magnoliaceae)木莲属的常绿阔叶植物，目前仅在广东龙门南昆山省级自然保护区发现1个种群，评价等级为极危(CR)。该研究通过溶剂萃取法得到了长梗木莲茎和叶的石油醚部位、乙酸乙酯部位、正丁醇部位和水部位，采用多种生物活性测试模型，对其不同萃取溶剂的部位进行了抗肿瘤、抗氧化及抗炎活性的评价。结果表明，长梗木莲抗肿瘤活性最优的是茎和叶的石油醚萃取部位，其次是乙酸乙酯部位，而正丁醇和水部位基本没有抗肿瘤活性；抗炎活性最优的是茎和叶的石油醚部位，IC₅₀值分别为(7.20±0.22)、(14.96±0.61)µg/mL；抗氧化活性最优的是茎和叶的乙酸乙酯部位，IC₅₀值分别为(5.12±0.13)、(0.46±0.09) µg/mL。该研究为首次对其茎和叶提取物的功能进行评价，可为其后期的开发利用提供物质基础和理论支持。

Abstract:

Manglietia longipedunculata, an evergreen broad-leaved plant belonging to the genus Manglietia of the Magnoliaceae family, has only been found in one population in the Nankunshan Provincial Nature Reserve in Longmen City, Guangdong Province. It was currently classified as critically endangered (CR). In this study, the petroleum ether fraction, ethyl acetate fraction, n-butanol fraction and water fraction from stems and leaves were obtained by solvent extraction. The anti-tumor, antioxidant and anti-inflammatory activities of different fractions were evaluated using multiple biological activity test models. The results showed that the petroleum ether fraction of the stems and leaves had the best anti-tumor activity, followed by the ethyl acetate fraction, while the n-butanol and water fractions had almost no anti-tumor activity. The petroleum ether fraction of the stems and leaves had the best anti-inflammatory activity, with IC₅₀ values of (7.20±0.22) and (14.96±0.61) µg/mL, respectively. The ethyl acetate fraction of the stems and leaves had the best antioxidant activity, with IC₅₀ values of (5.12±0.13) and (0.46±0.09) µg/mL, respectively. The functions of its stem and leaf extracts were evaluated at the first time, which could provide a material basis and theoretical support for its later development and utilization.