植物生长调节剂对青钱柳幼苗矮化及相关酶活性的影响

Effect of Plant Growth Regulators on Dwarfing and Related Enzyme Activities of Cylocarya paliurus Seedlings

摘要:

为探究施用植物生长调节剂对珍稀经济树种青钱柳(Cylocarya paliurus)生长和生理代谢的影响，选用矮壮素(C1: 500 mg/L、C2: 1 000 mg/L、C3: 1 500 mg/L、C4: 2 000 mg/L)、三碘苯甲酸(T1: 50 mg/L、T2: 100 mg/L、T3: 150 mg/L、T4: 200 mg/L)和芸苔素内酯(B1: 0.05 mg/L、B2: 0.1 mg/L、B3: 0.15 mg/L、B4: 0.2 mg/L)对1 a生青钱柳幼苗喷施叶面肥。结果表明，T1处理对幼苗苗高增长量的抑制作用和对地径增长量的促进效果最显著，B4处理对促进分枝数的增加最明显。3种植物生长调节剂均能促进幼苗的生物量积累，C2处理的幼苗总生物量积累最多。幼苗叶片过氧化物酶(POD)活性以T2处理最大，3种植物生长调节剂处理均能显著提高幼苗叶片吲哚乙酸氧化酶(IOD)活性，以C4处理最大。幼苗苗高与地径呈显著负相关，苗高与POD、IOD活性呈显著负相关，地径与2种酶活性呈显著正相关，POD与IOD活性呈显著正相关。3种植物生长调节剂均能有效抑制青钱柳幼苗的苗高增长，其中三碘苯甲酸表现出最强的矮化效果，而芸苔素内酯则在促进分枝方面表现最佳。中等浓度的生长调节剂处理显著促进幼苗生物量积累；POD和IOD活性的升高可促进根部生物量累积并抑制苗高增长。因此，根据栽培目的选择合适浓度的生长调节剂，可以有效改善青钱柳叶用林的结构和功能。

Abstract:

To explore the effects of plant growth regulators on growth and physiological metabolism of the rare economic tree species Cylocarya paliurus, foliar fertilizers of chlormequat chloride (C1: 500 mg/L, C2: 1000 mg/L, C3: 1500 mg/L, C4: 2000 mg/L), tridibenzyl (T1: 50 mg/L, T2: 100 mg/L, T3: 150 mg/L, T4: 200 mg/L), and brassinolide (B1: 0.05 mg/L, B2: 0.1 mg/L, B3: 0.15 mg/L, B4: 0.2 mg/L) were sprayed on one-year-old C. paliurus seedlings. The results showed that T1 treatment had the most significant inhibitory effect on the increase in seedling height and the most significant promoting effect on the increase in ground diameter. B4 treatment was the most effective in promoting the increase in the number of branches. All three plant growth regulators could promote the accumulation of seedling biomass, with the total biomass accumulation of seedlings being the highest under C2 treatment. The peroxidase (POD) activity in the leaves of seedlings was the highest under T2 treatment. All three plant growth regulator treatments could significantly increase the indoleacetic acid oxidase (IOD) activity in the leaves of seedlings, with the highest activity under C4 treatment. Seedling height was significantly negatively correlated with ground diameter, and seedling height was significantly negatively correlated with POD and IOD activities, while ground diameter was significantly positively correlated with the activities of the two enzymes, and POD activity was significantly positively correlated with IOD activity. All three plant growth regulators could effectively inhibit the increase in seedling height, among which tridibenzyl showed the strongest dwarfing effect, while brassinolide performed best in promoting branching. Moderate concentrations of growth regulators significantly promoted the accumulation of seedling biomass; the increase in POD and IOD enzyme activities could promote the accumulation of root biomass and inhibit the increase in seedling height. Therefore, choosing the appropriate concentration of growth regulators based on the cultivation purpose can effectively improve the structure and function of C. paliurus leaf-use forests.