毛车藤果实醇提物不同极性部位抗氧化活性研究

Study on the Antioxidant Activity of Different Polar Parts of the Ethanol Extract of Amalocalyx yunnanensis Fruit

摘要:

为了解毛车藤(Amalocalyx yunnanensis)果实中的抗氧化活性成分，通过体外抗氧化实验和噻唑蓝(MTT)比色法评估其果实醇提物中不同极性部位的抗氧化活性和细胞毒性，同时测定各部位的总酸和总酚含量，并分析其与抗氧化活性间的相关性，采用超高效液相色谱-四极杆轨道阱质谱联用仪(UHPLC-QE-MS)对最优抗氧化活性部位进行化学成分分析，明确抗氧化活性成分。结果表明，毛车藤果实醇提物及其不同极性部位均有一定的抗氧化活性且对LO₂细胞无毒性，其总酸和总酚含量与抗氧化活性呈正相关；其中乙酸乙酯部位抗氧化活性最强，总酸、总酚含量最高，分别为835 g/kg和14.49%；UHPLC- QE-MS分析共鉴定出98种化合物，其中黄酮类和酚酸类物质相对占比分别为30.21%和14.58%，可能是乙酸乙酯部位的主要活性成分。本研究可为毛车藤果实作为天然安全的抗氧化剂的开发提供基础。

Abstract:

To understand the antioxidant active components in fruits of Amalocalyx yunnanensis, the antioxidant activity and cytotoxicity of different polar fractions of the fruit’s ethanol extract were evaluated through in vitro antioxidant experiments and the MTT colorimetric method. Meanwhile, the total acid and total phenol contents of each fraction were determined, and their correlations with antioxidant activity were analyzed. The chemical components of the fraction with the best antioxidant activity were analyzed by ultra-high performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry (UHPLC-QE-MS) to identify the anti- oxidant active components. The results showed that the ethanol extract of A. yunnanensis fruits and its different polar fractions all had certain antioxidant activities and were non-toxic to LO₂ cells. The total acid and total phenol contents were positively correlated with antioxidant activity. Among them, the ethyl acetate fraction had the strongest antioxidant activity and the highest total acid and total phenol contents, which were 835 g/kg and 14.49%, respectively. Through UHPLC-QE-MS analysis, a total of 98 compounds were identified, among which flavonoids and phenolic acids accounted for 30.21% and 14.58%, respectively, which might be the main active components of the ethyl acetate fraction. Therefore, it could provide a basis for the development of A. yunnanensis fruits as natural and safe antioxidants.