Abstract:Understanding the interaction patterns between arbuscular mycorrhizal fungi and plant growth-promoting bacteria is of great significance for their combined application in crops. Eleven species with stress-resistant related traits were planted and the rhizosphere soil, root soil and plant root samples were collected for ITS amplification sequencing of fungal communities and 16S rRNA amplification sequencing of bacterial communities. The composition and abundance of Glomeromycota fungi in different groups of samples and the differences in their interaction patterns with bacteria were compared to infer the symbiotic rules of arbuscular mycorrhizal fungi and plant growth-promoting bacteria in the plant rhizosphere. The results showed that different spaces in the plant rhizosphere significantly affected the composition and abundance of Glomeromycota fungi. The relative abundance values of Glomeromycota fungi decreased in the order of planting soil, rhizosphere soil and plant roots (0.66%, 0.5% and 0.13%, respectively). Although the relative abundance value of Glomeromycota fungi in plant roots was the lowest, the positive correlation with the main bacterial groups was the highest among the three groups (29%, 52% and 74%), and the participation in the network of the main bacterial groups was also the highest, indicating the enhanced interaction between Glomeromycota fungi and bacteria in the roots. Finally, in the roots, Glomeromycota fungi were found to have significant positive correlations with plant growth-promoting bacteria (Arthrobacter, Rhizobium, Streptomyces), mycorrhizal helper bacteria (Arthrobacter, Bradyrhizobium, Paenibacillus, Rhizobium, Streptomyces) and some other bacterial genera. Among them, Glomeromycota fungi (represented by Glomerales and Diversisporales) may interact with the representative populations of the plant-related bacterial genera Bradyrhizobium and Rhizobium, which is worthy of further verification.