11种抗逆植物根际球囊菌门真菌和细菌的互作特征

Rhizosphere Glomeromycota-Bacteria Interaction Pattern of 11 Stress-resistant Species

摘要:

了解丛枝菌根真菌和植物促生细菌的互作规律对于它们在农作物上的联用具有重要意义。该研究种植了11种具有抗逆相关性状的植物，并收集其种植土、根际土和植物根系样品进行真菌群落ITS和细菌群落16S rRNA的扩增子测序，比较不同组别样品中球囊菌门真菌的组成、丰度以及与细菌互作模式的差异来推测丛枝菌根真菌和植物促生细菌在植物根际的共生规律。结果表明，植物根际的不同空间显著影响球囊菌门真菌的组成和丰度，在种植土、根际土和植物根系中，球囊菌门真菌的相对丰度值依次递减(0.66%、0.5%和0.13%)。尽管在植物根系中的相对丰度值最低，球囊菌门真菌与细菌主要类群的正相关性却是3个组别中最高的(29%、52%和74%)，而且对于细菌主要类群组成的网络的参与度也最高，表现出球囊菌门真菌和细菌在根系中互作的增强。在根系中发现球囊菌门真菌与植物促生细菌(Arthrobacter、Rhizobium、Streptomyces)、菌根辅助细菌(Arthrobacter、Bradyrhizobium、Paenibacillus、Rhizobium、Streptomyces)和其他的一些细菌属群存在显著的正相关关系。其中，球囊菌门真菌(以球囊霉目和多孢囊霉目为代表)与植物紧密相关的细菌属群慢生根瘤菌属和赖氏菌属的代表种群可能存在互作，值得进一步验证。

Abstract:

Understanding the interaction patterns between arbuscular mycorrhizal fungi and plant growth-promoting bacteria is of great significance for their combined application in crops. Eleven species with stress-resistant related traits were planted and the rhizosphere soil, root soil and plant root samples were collected for ITS amplification sequencing of fungal communities and 16S rRNA amplification sequencing of bacterial communities. The composition and abundance of Glomeromycota fungi in different groups of samples and the differences in their interaction patterns with bacteria were compared to infer the symbiotic rules of arbuscular mycorrhizal fungi and plant growth-promoting bacteria in the plant rhizosphere. The results showed that different spaces in the plant rhizosphere significantly affected the composition and abundance of Glomeromycota fungi. The relative abundance values of Glomeromycota fungi decreased in the order of planting soil, rhizosphere soil and plant roots (0.66%, 0.5% and 0.13%, respectively). Although the relative abundance value of Glomeromycota fungi in plant roots was the lowest, the positive correlation with the main bacterial groups was the highest among the three groups (29%, 52% and 74%), and the participation in the network of the main bacterial groups was also the highest, indicating the enhanced interaction between Glomeromycota fungi and bacteria in the roots. Finally, in the roots, Glomeromycota fungi were found to have significant positive correlations with plant growth-promoting bacteria (Arthrobacter, Rhizobium, Streptomyces), mycorrhizal helper bacteria (Arthrobacter, Bradyrhizobium, Paenibacillus, Rhizobium, Streptomyces) and some other bacterial genera. Among them, Glomeromycota fungi (represented by Glomerales and Diversisporales) may interact with the representative populations of the plant-related bacterial genera Bradyrhizobium and Rhizobium, which is worthy of further verification.