不同栽培基质下青钱柳实生苗生长和生理响应特征分析

Analysis of Growth and Physiological Response Characteristics of Cyclocarya paliurus Seedlings Under Different Cultivation Substrates

摘要:

为筛选青钱柳(Cyclocarya paliurus)高质量实生苗培育的适宜栽培基质，采用容器育苗，测定4种栽培基质(J1、J2、J3和J4)对实生苗成活率、生长指标和生理指标的影响。结果表明，J1培育的实生苗成活率为100%，是J2培育的2.25倍；且在株高、叶面积、地径等生长指标均优于其他3种基质。J2培育的苗内含水量达到67.25%，可溶性糖含量仅为2.33%，且苗内Ca含量最高，而Na含量最低。J4培育的叶内K和Mg含量最高；J1培育的叶内4种矿质元素的转运系数显著低于其他3种基质的。与J2相比，J1培育的苗叶内SOD、POD、CAT活性和MDA含量分别降低了92.36%、80.98%、87.21%和81.51%；PPO活性是J2的13.30倍。通过隶属函数和主成分分析，函数值大小依次为J1 > J4 > J3 > J2，且J1对实生苗培育的贡献率为89.71%；Mantel’s分析表明实生苗成活率、生长指标与含水量、SOD活性具有显著相关性。因此，按10:1体积比混合泥炭土和珍珠岩的基质是青钱柳实生苗适宜的栽培基质，培育的实生苗成活率高，且植株生长健壮。

Abstract:

To screen the suitable cultivation substrate for the high-quality seedling production of Cyclocarya paliurus, container seedling cultivation was adopted to determine the effects of four cultivation substrates (J1, J2, J3 and J4) on the survival rate, growth indicators and physiological indicators of seedlings. The results showed that the survival of seedlings cultivated in J1 was 100%, which was 2.25 times that of J2. Moreover, the seedlings cultivated in J1 had better growth indicators, such as plant height, leaf area and ground diameter than those in the other three substrates. The water content of seedlings cultivated in J2 reached 67.25%, and the content of soluble sugar was only 2.33%. The content of Ca in the seedlings was the highest, while the content of Na was the lowest. The content of K and Mg in the leaf of seedlings cultivated in J4 was the highest. The translocate factor of four mineral elements in the leaves cultivated in J1 was significantly lower than that in the other three substrates. Compared with J2, the activities of SOD, POD and CAT and the content of MDA in the leaves of seedlings cultivated in J1 decreased by 92.36%, 80.98%, 87.21% and 81.51%, respectively, while the activity of PPO was 13.30 times that of J2. Through the membership function and principal component analysis, the function values were in the order of J1 > J4 > J3 > J2, and the contribution rate of J1 to the seedling cultivation was 89.71%. Mantel's analysis showed that the survival and growth indicators of seedlings were significantly correlated with water content and SOD activity. Therefore, the substrate mixed with peat soil and perlite at a volume ratio of 10:1 is a suitable cultivation substrate for C. paliurus seedlings, which has a high survival rate and vigorous plant growth.