基质和植物生长调节剂对猪血木组培苗、实生苗插穗生根的效应

Effects of Matrix and Plant Growth Regulators on Cutting Rooting from Tissue Culture Seedlings and Seedlings of Euryodendron excelsum

摘要:

为建立猪血木(Euryodendron excelsum)高效扦插快繁体系，该研究以移栽3 a的猪血木组培苗的1 a生侧芽、2 a生侧枝不同节位及实生苗成年株1 a生侧芽为材料，研究基质种类和植物生长调节剂浸泡时间对插穗生根的影响。结果表明，插穗生根方式为混合生根型，组培幼苗的生根率显著高于成年株，蛭石:珍珠岩体积比为2:1时最适宜生根，经吲哚丁酸(IBA)处理后的插穗生根率远高于萘乙酸(NAA)处理。组培幼苗插穗经1 500 mg/L IBA浸泡20 min的生根率和平均根长最高，分别为96.67%和0.97 cm；成年株插穗经过2 000 mg/L IBA浸泡10 s的生根率最高，为34.44%，平均生根数为4.22，平均根长为0.53 cm。组培幼苗2 a生侧枝顶端1~3节的生根率最高，为46.67%。采用组培幼苗作为插穗母株，成功率高，可以提高繁殖效率，降低生产成本。

Abstract:

In order to establish an efficient and rapid propagation system for cuttings of Euryodendron excelsum, the effects of substrate type and soaking time of plant growth regulator on rooting of cuttings were studied by using 1-year-old lateral bud and 2-year-old lateral bud of 3-year-old tissue culture seedlings and 1-year-old lateral bud of adult seedlings. The results showed that the rooting mode of cuttings was mixed rooting type, the rooting rate of tissue culture seedlings was significantly higher than that of adult plants. The rooting rate of cuttings treated with indolebutyric acid (IBA) was much higher than that treated with naphthalene acetic acid (NAA) on medium with the volume ratio of vermiculite to perlite for 2:1. The rooting rate and average root length of cutting tissue culture seedlings soaked in 1 500 mg/L IBA for 20 min were the highest, which were 96.67% and 0.97 cm, respectively. The rooting rate of adult cuttings soaked in 2 000 mg/L IBA for 10 s was the highest (34.44%), the average root number was 4.22, and the average root length was 0.53 cm. The rooting rate was the highest (46.67%) in the top 1-3 segments of the 2-year-old lateral branches. So, the tissue culture seedlings as cuttings mother plant had a high success rate, could improve the reproduction efficiency and reduce the production cost.