Abstract:In order to investigate the composition of metabolites in the leaves of a new tea strain of ‘Chunlv’, the metabolites in the leaves of ‘Chunlv’ and mother tree (‘Fuyun 6’) were analyzed by using LC-MS and GC-MS. The results showed that 50 metabolites with significant differences (VIP>1.0) were identified by GC-MS, mainly including sugars and amino acids, among which 9 were up-regulated and 41 were down-regulated. The chlorogenic acid content had the greatest difference. There were 297 metabolites with significant differences (VIP>1.0) identified by LC-MS, 176 up-regulated and 121 down-regulated, mainly concentrated in polyphenols, alkyds and sugars, among which caffeine had the greatest difference. Pathway enrichment analysis showed that the differential metabolites were mainly enriched in the biosynthesis pathway of flavonoids and flavonols, the biosynthesis pathway of arginine, the biosynthesis pathway of unsaturated fatty acids, and the mutual conversion pathway of pentose and glucuronic acid. Variable contribution analysis of OPLS-DA showed that most of the potential biomarkers were related to energy metabolism and quality, linoleic acid, oleic acid, phytol, hydroxypiperidolic acid, ribosol, xylose were related to energy metabolism, chlorogenic acid, tryptophan, caffeine, theophylline and so on were closely related to quality. The analysis of metabolites and metabolic pathways provided a theoretical basis for the quality formation and variety breeding of ‘Chunlv’.
