紫玉兰‘红元宝’MIKC型MADS-box家族鉴定及其在多季成花中的作用探析

Identification of MIKC-type MADS-box Gene Family of Yulania liliiflora ‘Hongyuanbao’ and Its Function in Multiseasonal Flower Production

摘要:

为了解紫玉兰(Yulania liliiflora) MADS-box基因家族成员的功能和表达模式，基于‘红元宝’ (‘Hongyuanbao’)转录组数据，对其MIKC型MADS-box基因家族成员进行鉴定、理化性质、系统进化和保守结构域分析。结果表明，共鉴定出19个MIKC型MADS-box基因家族成员，均定位于细胞核中且拥有共同的MADS或K-box保守结构域。大部分MlMADSs在花器官建成中发挥功能，且MlAGL6和MlAGL12-c作为蛋白质互作网络的中心节点可能发挥关键作用。MlMADS-box成员在雄蕊、花芽和茎等组织中均有较高的表达水平，在花器官形成和花发育过程中发挥重要作用，并筛选到调控紫玉兰‘红元宝’ 2次成花的候选关键基因MlAGL6。这为探索MIKC型MADS-box基因在紫玉兰‘红元宝’生殖发育过程的功能提供数据支持。

Abstract:

In order to understand the function and expression patterns of the MADS-box gene family in Yulania liliiflora, based on the transcriptomic data of ‘Hongyuanbao’, the members of MIKC MADS-box gene family were identified, their physical and chemical properties, phylogenetic evolution and conserved domain were analyzed. The results showed that a total of 19 MIKC MADS-BOX gene family members were identified, all of which were located in the nucleus and shared common MADS or K-box conserved domains. Most MlMADSs played role in floral organogenesis, and MlAGL6 and MlAGL12-c might be important nodes in the protein interaction network. MlMADS-box members had high expression in stamen, flower bud and stem tissues, and play an important role in flower organ formation and flower development. The candidate key gene MlAGL6 was screened regulating the secondary flower formation of ‘Hongyuanbao’. These would provide data support for exploring the function of MIKC MADS-box gene in the reproductive development of Y. liliiflora ‘Hongyuanbao’.