盐胁迫对木奶果苗抗逆生理学影响

Effect of Salt Stress on Anti-adversity Physiology of Baccaurea ramiflora Seedlings

摘要:

为了解盐胁迫对木奶果生长的影响，研究了不同土壤盐浓度(0.05%、0.1%、0.15%和0.2%)对木奶果(Baccaurea ramiflora)幼苗抗逆生理机制的影响。结果表明，盐胁迫激发了木奶果幼苗的适应性，尤其是在低盐浓度(0.05%)下，可溶性糖含量和过氧化物酶活性显著增加。盐浓度达到0.1%时，可溶性蛋白含量显著增加；0.15%的盐浓度下，丙二醛含量显著上升；而游离脯氨酸和类黄酮含量在0.2%盐浓度下才表现出显著累积。盐处理的初期阶段(第1周)，过氧化物酶活性迅速响应，其他指标在2周后显著变化。随处理时间的延长，部分指标如游离脯氨酸和可溶性蛋白含量及过氧化物酶活性呈先升后降的趋势。这对木奶果耐盐性育种和耐盐作物开发具有重要意义。

关键词:盐胁迫;木奶果;渗透调节物质;活性氧防御酶

Abstract:

In order to understand the effects of salt stress on the growth of Baccaurea ramiflora seedlings, the physiological mechanism of stress resistance were studied treated with different soil salt concentrations (0.05%, 0.1%, 0.15% and 0.2%). The results showed that salt stress stimulated the adaptability of B. ramiflora seedlings, especially at low salt concentration (0.05%), soluble sugar content and peroxidase activity increased significantly. When the salt concentration reached 0.1%, the soluble protein content increased significantly. The content of malondialdehyde increased significantly at 0.15% salt concentration. The content of free proline and flavonoids showed significant accumulation at 0.2% salt concentration. In the initial phase (1^st week) of salt treatment, peroxidase activity responded rapidly, and other indicators changed significantly after 2 weeks. Some indexes, such as free proline and soluble protein contents and peroxidase activity, increased at first and then decreased with the treatment time. So, it was of great significance for breeding salt-tolerance of B. ramiflora and developing salt-tolerant crops.

Key words:Salt stress;Baccaurea ramiflora;Osmotic regulation substance;Active oxygen defense enzyme