光叶兔耳风石油醚部位化学成分和抗菌活性研究

Chemical Constituents from Petroleum Ether Fractions of Ainsliaea glabra and Antibacterial Activities

摘要:

为了解兔耳风(Ainsliaea glabra)的化学成分，采用硅胶、凝胶(Sephadex LH-20)柱色谱、半制备高效液相色谱等方法，从其90%乙醇提取物的石油醚萃取部位分离了10个化合物，根据波谱数据分别鉴定为松柏烯醇二异戊酸酯 (1)、3β-hydroxy- 11α,13-dihydro-costunolide (2)、matricarin (3)、乙酰伪蒲公英甾醇 (4)、白桦脂醇 (5)、27-hydroxyolean-12-en-28-oic acid (6)、morolic acid acetate (7)、异黑麦草内酯 (8)、苯甲酸丁酯 (9)和E-phytenal (10)，其中化合物1为新结构化合物，化合物3和7首次在兔耳风属中分离得到，化合物2~7、9均为首次在光叶兔耳风报道。抗菌活性测试结果表明，化合物1、2、8对金黄色葡萄球菌有一定抑制作用，MIC值分别为31.2、25.0、21.8 μg/mL，化合物1和3对白色念珠菌有潜在抑制作用，MIC值分别为1.9和3.9 μg/mL。

Abstract:

In order to understand the chemical composition of Ainsliaea glabra, ten compounds were isolated from petroleum ether extraction fraction of 90% ethanol extract by silica gel, sephadex LH-20 gel column chromatography, semi-preparative high-performance liquid chromatography. Based on spectral data, their structures were identified as pinerenol diisovalerate (1), 3β-hydroxy-11α,13-dihydro-costunolide (2), matricarin (3), taraxasteryl acetate (4), betulyl alcohol (5), 27-hydroxyolean-12-en-28-oic acid (6), morolic acid acetate (7), (-)-loliolide (8), butyl benzoate (9), and E-phytenal (10). Compound 1 was a new structural compound. Compounds 3 and 7 were isolated from Ainsliaea genus for the first time. Compounds 2-7 and 9 were first reported in the A. glabra. The results of antibacterial activity test showed that compounds 1, 2 and 8 had certain inhibitory effects on Staphylococcus aureus with MIC values of 31.2, 25.0, and 21.8 μg/mL, and compounds 1 and 3 had potential inhibitory effect on Candida albicans with MIC values of 1.9 and 3.9 μg/mL, respectively.