木薯FER基因家族的全基因组鉴定和表达分析

Genome-wide Identification and Expression of FER Gene Family in Manihot esculenta

摘要:

植物铁蛋白(Ferritin, FER)既能存储铁，又能响应各种非生物胁迫。该研究基于全基因组水平对木薯(Manihot esculenta)的FER基因家族进行分析，结果表明, 从木薯中共鉴定到4个FER基因，根据系统发育树将木薯FER基因划分为2支，所有成员均包含Euk_Ferritin的功能结构域并位于叶绿体内。木薯FERs基因位于LG7~LG10染色体上；基因共线性分析表明，共有3对潜在的复制基因对，无串联重复事件；Ka/Ks值表明，MeFER同源基因经过了纯化选择；该家族含有响应激素和胁迫诱导的顺式作用元件；qRT-PCR分析表明，MeFER基因的表达具有组织特异性，MeFER4基因响应多种胁迫，且在干旱胁迫下响应最为显著。该研究为木薯FER基因家族的功能研究奠定了基础。

关键词:木薯;FER基因;生物信息学分析;非生物胁迫

Abstract:

Plant ferritin (FER) can store iron and respond to various abiotic stresses. The FER gene family of cassava (Manihot esculenta) was analyzed based on a genome-wide level. The results showed that four FER genes had been identified from cassava, named MeFERs, encoded MeFERs could be divided into two clades based on phylogenetic tree analysis. All members contained Euk_Ferritin functional domain, and located in the chloroplast. The MeFERs genes located in chromosome LG7-LG10, respectively. Gene collinearity analysis showed that there were three potential duplicate gene pairs without tandem duplication events. The Ka/Ks value indicated that the homologous gene of MeFER was purified and selected. The family contained cis-acting elements in response to hormones and stress induction. Moreover, qRT-PCR analysis showed that the expression of MeFERs gene was tissue-specific, and MeFER4 gene responded to various stresses, with the most significant response under drought stress. Therefore, these would provide a foundation for future functional studies of the cassava FER gene family.

Key words:Manihot esculenta;FER gene;Bioinformatics analysis;Abiotic stress