金钗石斛中一个新的木防己毒烷型倍半萜

One New Picrotoxane-type Sesquiterpene from Dendrobium nobile

摘要:

为了解金钗石斛乙醇提取物正丁醇部位的化学成分，通过硅胶、凝胶、反相硅胶和高效液相等各种色谱技术进行分离纯化得到3个化合物，并根据核磁共振、质谱等波谱手段确定其结构分别为2,11-epoxy-11,13-dihydroxypicrotoxano-3(15)- lactone (1)、2,4,6-三甲氧基苯-1-O-β-d-葡萄糖苷 (2)和3-methylbutan-1-ol β-d-glucopyranoside (3)。其中化合物1是一个新的木防己毒烷型倍半萜，该类化合物是金钗石斛中具有代表性的倍半萜类型。所有化合物均没有α-葡萄糖苷酶和酪氨酸酶抑制活性，同时化合物1也没有神经保护活性。

关键词:金钗石斛;化学成分;倍半萜;新化合物

Abstract:

In order to study the chemical constituents in butanol fraction of ethanol extract of Dendrobium nobile, column chromatographic techniques including silica gel, Sephadex LH-20, revised silica gel and HPLC were used to separate the compounds, and spectroscopic techniques including NMR and MS were implied to determine the structures. Totally 3 compounds were isolated, and their structures were identified as 2,11-epoxy-11,13-dihydro-xypicrotoxano-3(15)-lactone (1), 2,4,6-trimethoxyphenol-1-O-β-d-glycoside (2), 3-methylbutan-1-ol β-d-gluco-pyranoside (3). Compound 1 was a new picrotoxane-type sesquiterpene, which is one of the representative components in D. nobile. However, none of the compounds showedα-glucosidase and tyrosinase inhibitory activity, none of them showed activity, and compound 1 show no neuroprotective activity either.

Key words:Dendrobium nobile;Chemical constituent;Sesquiterpene;New compound