异叶三宝木的二萜成分及抗菌活性研究

Chemical Constituents and Antibacterial Activities of Trigonostemon heterophyllus

摘要:

为研究异叶三宝木(Trigonostemon heterophyllus)的二萜成分及其抗菌活性，采用硅胶柱层析、凝胶柱层析、高效液相色谱对萃取物进行分离纯化，结合现代波谱技术对所得化合物进行结构鉴定，并通过牛津杯法和2倍稀释法检测化合物对革兰氏阴性菌大肠杆菌(Escherichia coli)以及革兰氏阳性菌肺炎双球菌(Pneumococcus)的生长抑制活性和MIC值。从异叶三宝木中分离得到了6个化合物，包括5个二萜类， 1个木脂素类，分别鉴定为trigonochinene E (1)、neoboutomannin (2)、6,9-O- dedimethyltrigonostemone (3)、stelltian B (4)、3,4-secosonderianol (5)、biondinin A (6)。化合物1、2、3、5对大肠杆菌有抑制作用，MIC值分别为9.375、18.75、18.75、18.75 μg/mL。化合物2、3、4、6为首次从该种植物中分离得到，化合物4为首次从该属植物中分离得到。除化合物4外，其他化合物都有一定的抗菌活性，且化合物1、2、3对大肠杆菌抑制作用强于阳性对照硫酸卡那霉素。

Abstract:

To understand the chemical constituents and antibacterial activities of Trigonostemon heterophyllus, six compounds, including five diterpenoids and one lignan, were isolated and purified by silica gel column chromate- graphy, gel column chromatography and high performance liquid chromatography. Based on spectroscopic data, their structures were identified as trigonochinene E (1), neoboutomannin (2), 6,9-O-dedimethyl trigonostemone (3), stelltian B (4), 3,4-secosonderianol (5), biondinin A (6). The inhibitory activity and MIC values of compounds against a Gram-negative bacterium, Escherichia coli, and a Gram-positive bacterium, Pneumococcus, were detected by Oxford Cup method and double dilution method. Compounds 1, 2, 3 and 5 had inhibitory effects on Escherichia coli with MIC values of 9.375, 18.75, 18.75 and 18.75 μg/mL, respectively. Compounds 2, 3, 4, and 6 were isolated from T. heterophyllus for the first time, and compound 4 was isolated from Trigonostemon genus for the first time. Except compound 4, all the other compounds had certain antibacterial activity, and compounds 1, 2 and 3 had stronger inhibitory effect on Escherichia coli than Kanamycin sulfate positive control.