振动对三角梅苞片脱落和乙烯生成的影响

Effects of Vibration on Shedding and Ethylene Production in Bougainvillea Glabra Bracts

摘要:

为探究振动对盆栽三角梅苞片脱落和乙烯生成的影响，以‘马尼拉小姐’(Bougainvillea × buttiana ‘Miss Manila’)盆栽三角梅为试验材料，测定在振动、密闭环境和振动+密闭环境处理下苞片的脱落率以及处理后4~24 h内的乙烯释放量、1-氨基环丙烷-1-羧酸(ACC)含量、ACC合成酶(ACS)活性、ACC氧化酶(ACO)活性、乙烯合成相关基因的相对表达量。结果表明:经振动+密闭环境和振动处理后7 d三角梅苞片脱落率显著高于对照(无处理)，苞径长0.5~1.4 cm的苞片(S1)和苞径长1.5~2.4 cm的苞片(S2)的乙烯释放量、ACC含量、ACS和ACO活性均显著高于对照，随着处理后时间的变化，总体呈先上升后下降的变化趋势。苞片S1和S2的BsSAMS、BsACS和BsACO基因相对表达量显著上升，与ACS活性和ACO活性的变化一致，总体呈先上升后下降的变化趋势。盆栽三角梅在受到振动后苞片的脱落率、乙烯生成、乙烯生物合成关键酶活性、乙烯合成酶基因BsSAMS、BsACS、BsACO的相对表达量均显著上升，在振动的基础上，密闭环境对苞片脱落、乙烯生物合成、乙烯合成酶基因的表达具有促进作用。

关键词:三角梅;振动处理;乙烯生成;基因表达

Abstract:

In order to explore the effects of vibration on the shedding of bougainvillea glabra bracts and ethylene production in potted bougainvillea glabra, using the ‘Miss Manila’(Bougainvillea × buttiana ‘Miss Manila’)potted bougainvillea glabra as the test material, the bract shedding rate after treatments was measured under vibration , airtight environment and vibration and airtight environment treatments. The ethylene release, the content of 1-aminocyclopropane-1-carboxylic acid (ACC), the activity of ACC synthesis (ACS), the activity of ACC oxidase (ACO), relative expression of ethylene synthesis related genes were measured within 4 to 24h after treatment. The results showed that the shedding of bracts of potted bougainvillea glabra was significantly higher than that of the control (without treatment) within 7 days after vibration and airtight environment treatment and vibration treatment, and the ethylene release, ACC content, ACS activity and ACO activity of bracts (S1) with bract diameter of 0.5-1.4 cm and bract (S2) with bract diameter of 1.5-2.4 cm are all significantly higher than that of the control. With the change of time after treatment, it showed a trend of first increasing and then decreasing. The relative expression of BsSAMS, BsACS, BsACO genes in bracts S1 and S2 increased significantly, and its change trend was consistent with the change of ACS activity and ACO activity, which showed a trend of first increasing and then decreasing. The shedding of bracts, ethylene production, key enzyme activities of ethylene biosynthesis, the expression of ethylene synthesis genes BsSAMS, BsACS, BsACO all increase significantly after vibration in potted bougainvillea glabra. On the basis of vibration, a closed environment can promote bract shedding, ethylene biosynthesis, ethylene synthesis gene expression.

Key words:Bougainvillea glabra; vibration treatment; ethylene production; gene expression