油茶DELLA基因的克隆和表达分析

Cloning and Expression Analysis of DELLA Genes in Camellia oleifera

摘要:

为了解油茶(Camellia oleifera)中DELLA基因功能及其表达特性，采用PCR技术从‘长林4号’油茶中克隆了5个DELLA基因，命名为CoDELLA1~CoDELLA5，对其编码的5个CoDELLA蛋白进行生物信息学分析，并对5个DELLA基因的表达模式以及激素响应活性进行了分析。结果表明，5个CoDELLA基因的编码区长度分别为1 791、1 875、1 848、1 593和1 581 bp,分别编码597、625、616、531和527个氨基酸。5个CoDELLA蛋白的氨基酸序列相似度较高，丝氨酸残基为主要的潜在磷酸化位点，CoDELLA蛋白N端均含有典型的DELLA结构域。不同物种中DELLA蛋白的系统发育存在差异，CoDELLA与茶树的CsDELLA同源性最高。CoDELLA基因在油茶不同组织中的表达也存在差异，且赤霉素和独脚金内酯等多种激素和非生物胁迫对其表达具有调控作用。CoDELLA基因可能在油茶的生长发育和非生物胁迫响应中发挥重要作用。

关键词:油茶;DELLA;基因;生物信息学;基因表达

Abstract:

To reveal the function and expression pattern of DELLA genes in Camellia oleifera, five DELLA genes, named CoDELLA1, CoDELLA2, CoDELLA3, CoDELLA4 and CoDELLA5, were cloned from 'Changlin 4' by PCR technique. The bioimformation of 5 encoding CoDELLA were analyzed, as well as expression patterns of 5 CoDELL and hormone response activities. The results showed that the CDS length of five CoDELLA genes were 1 791, 1 875, 1 848, 1 593 and 1 581 bp, encoding 597, 625, 616, 531 and 527 amino acids, respectively. The amino acid sequences of the five CoDELLA proteins were highly similar, and serine residues were the main potential phosphorylation sites. The N-terminal of CoDELLA proteins all contained typical DELLA domains. There were differences in the phylogeny of DELLA proteins from different species, CoDELLA had the highest homology with CsDELLA in C. sinensis. The expression of CoDELLA gene in different tissues was also different, which was regulated by various hormones and abiotic stresses such as gibberellin and aurolactone. Therefore, it was suggested that CoDELLA genes might play important role in the growth of C. oleifera and response to abiotic stress.

Key words:Camellia oleifera;DELLA;Gene;Bioinformatic;Gene expression