首页 > 过刊浏览>2023年第31卷第5期 >623-633. DOI:10.11926/jtsb.4663
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景宁木兰TCP家族鉴定及遮阴胁迫表达模式分析
作者:
基金项目:

浙江省“十四五”育种专项(2021C02071-3)资助


Identification of TCP Family in Magnolia sinostellata and Expression Patterns of TCPs Under Shading Stress
Author:
  • REN Mingjie

    REN Mingjie

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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  • LU Danying

    LU Danying

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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  • WU Chao

    WU Chao

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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  • ZHU Senyu

    ZHU Senyu

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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  • CHEN Xiaojun

    CHEN Xiaojun

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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  • WU Yufeng

    WU Yufeng

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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  • SHEN Yamei

    SHEN Yamei

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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  • MA Jingjing

    MA Jingjing

    School of Landscape Architecture, Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang A&F University, Hangzhou 311300, China
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Fund Project:

The Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(Grant No. 2021C02071-3)

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    摘要:

    为明确TCP转录因子家族在景宁木兰(Magnolia sinostellata)遮阴胁迫中的作用，通过模拟自然生境对景宁木兰3 a生嫁接苗进行遮阴处理，基于转录组分析TCP转录因子相关信息，利用转录组表达量和qRT-PCR分析遮阴胁迫下的表达模式。结果表明，从景宁木兰转录组中筛选出12个TCPs基因，编码蛋白长度为228~558个氨基酸，分子量为23.7~52.7 kDa，TCPs蛋白均为不稳定蛋白、亲水性蛋白；TCPs均定位于细胞核，MsTCP20-a/b蛋白还定位于叶绿体。TCPs成员分为2大类，Class Ⅰ包含10个TCPs成员，Class Ⅱ包含2个TCPs成员。所有成员均含有TCP保守结构域，亲缘关系较近的成员具有相似的保守基序；克隆得到TCPs启动子序列，其序列含有大量光响应和激素响应元件。在遮阴处理后景宁木兰TCPs基因存在差异表达，其中MsTCP9-a与MsTCP2在遮阴后显著上升，MsTCP8、MsTCP9-b、MsTCP7-a、MsTCP15在遮阴后显著下降。因此，景宁木兰TCPs可能在响应遮阴胁迫过程中发挥重要功能。

    Abstract:

    In order to investigate the role of TCP transcription factors family of 3-year-old Magnolia sinostellata grafted seedlings under simulated natural shading, the bioinformation of TCP transcription factors were analyzed based on transcriptome data, as well as their expression patterns under shading stress. The results showed that 12 TCPs were identified from the transcriptome data of M. sinostellata, encoding 228-558 amino acids with protein size of 23.7-52.7 kDa. All of 12 TCPs proteins were unstable and hydrophilic. The 12 TCPs located in cell nucleus, and MsTCP12-a/b also located in chloroplast. MsTCPs could be divided into two types, Class I contained 10 TCP members, and Class II with two TCP members. All members contained TCP conserved domains and the closely TCP members had similar conserved motifs. Meanwhile, the TCP promoter sequences of M. sinostellata were cloned and analyzed, which contained a large number of light response elements and hormone response elements. The MsTCPs were differentially expressed under shading treatment. MsTCP9-a, MsTCP2 were significantly up-regulated and MsTCP8, MsTCP9-b, MsTCP7-a, MsTCP15 were significantly down-regulated. Therefore, MsTCPs might play an important role in response to shade stress.

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任明杰,陆丹迎,吴超,朱森宇,陈小珺,邬宇峰,申亚梅,马晶晶.景宁木兰TCP家族鉴定及遮阴胁迫表达模式分析[J].热带亚热带植物学报,2023,31(5):623~633

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  • 收稿日期:2022-04-28
  • 最后修改日期:2022-06-15
  • 录用日期:2022-06-16
  • 在线发布日期: 2023-09-26
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