米碎花的酚苷类化合物及其鼻咽癌细胞毒活性

Phenolic Glycosides from Eurya chinensis and Their Cytotoxic Activities Against Nasopharyngeal Carcinoma Cells

摘要:

为了解米碎花(Eurya chinensis)的化学成分及其生物活性，运用多种色谱技术从其乙醇提取物分离得到11个化合物, 并对化合物进行体外抗鼻咽癌细胞增殖活性评价。经波谱数据分析，分别为异落新妇苷(1)、3,5,7-三羟基色原酮-3-O-α-L-鼠李糖苷(2)、1-O-反式-桂皮酰基-β-D-葡萄糖(3)、1-O-(4-羟基苯乙基)-6-O-反式-桂皮酰基-β-D-葡萄糖(4)、eutigoside D (5)、1-O-(3,4-二羟基苯乙基)-6-O-反式-香豆酰基-β-D-葡萄糖(6)、eutigoside A (7)、1-O-(4-羟基苯乙基)-6-O-反式-咖啡酰基-β-D-葡萄糖(8)、grayanoside A (9)、1-O-(4-羟基苯乙基)-6-O-(4-羟基苯甲酰基)-β-D-葡萄糖(10)、3-O-β-D-葡萄糖基-4-羟基-苄基苯甲酸酯(11)。其中，化合物4为首次从天然来源获得，化合物2~4和8~11均为首次从该属植物中分离得到。MTT法表明，化合物10具有中等抑制5-8F细胞增殖活性。

Abstract:

To clarify the chemical constituents and their biological activities of Eurya chinensis, eleven compounds were isolated from its MeOH extract by various column chromatography methods, and the anti-proliferative activity of compounds against human nasopharyngeal carcinoma cells (5-8F) were evaluated. Basis on spectral data, their structures were identified as isoastinlbin (1), 3,5,7-trihydroxychromone-3-O-α-L-arabino-pyranoside (2), 1-O-trans-cinnamoyl-β-D-glucopyranose (3), 2-(4-hydroxyphenyl)ethyl-6-O-(E)-cinnamoyl-β-D-glucopyranoside (4), eutigoside D (5), 6-O-coumaroyl-1-O-[2-(3,4-dihydroxyphenyl)ethyl]-β-D-glucopyranoside (6), eutigoside A (7), 2-(4-hydroxypheny)ethyl-[6-O-(E)-caffeoyl]-β-D-glucopyranoside (8), grayanoside A (9), 4-hydroxyphenylethyl-1-O-β-D-[6'-O-(4-hydroxybenzoyl)]-glucopyranoside (10), benzyl-4'-hydroxybenzoyl-3'-O-β-D-glucopyranoside (11). Compound 4 was isolated from natural resources for the first time, while the compounds 2- 4, 8- 11 were obtained from the genus Eurya for the first time. The compound 10 showed moderate inhibitory activity on the proliferation of 5-8F cells by MTT method.