白化菠萝蜜茎2个蛋白激酶和4个转录因子家族分析

Analysis on 2 Protein Kinases and 4 Transcription Factors Families in Albino Artocarpus heterophyllus Stems

摘要:

为探究蛋白激酶(PKs)和转录因子(TFs)在白化菠萝蜜(Artocarpus heterophyllus)幼苗茎次生生长中的表达变化，基于转录组数据对其差异表达基因(DEGs)进行预测及分类，并对挑选出的2个PKs和4个TFs家族构建系统进化树。结果表明, 胞质类受体激酶(RLCK)-VIII家族的DEGs上下调表达各4个，亮氨酸富集重复类受体激酶(LRR-RLK)-X家族Xa和Xb-2分支中的DEGs均下调表达，Xb-1中的均上调，TCP家族的20个DEGs中有15个上调表达，zf-HD和GRF家族中的大多数DEGs上调表达，Alfin-like家族中的DEGs均下调表达。因此，这表明6个家族可能在菠萝蜜茎的次生生长过程和应对非生物胁迫中发挥重要作用。

Abstract:

Protein kinases (PKs) and transcription factors (TFs) are important components of signal transduction, which are involved in the secondary growth of stems. To explore their expression changes in stem secondary growth of albino Artocarpus heterophyllus seedlings, the differentially expressed genes (DEGs) of PKs and TFs were predicted and classified based on transcriptome data, and phylogenetic trees were constructed for 2 PKs and 4 TFs families. The results show that the number of up- and down-regulated DEGs in the receptor-like cytoplasmic kinase (RLCK)-VIII family was 4 each. All of DEGs in Xa and Xb-2 branches in leucine-rich repeat receptor kinase (LRR-RLk)-X family were down-regulated, while those in Xb-1 branch were up-regulated. Among 20 DEGs in TCP family, 15 were up-regulated in AAS. And most genes in zf-HD and GRF families were up-regulated, while all DEGs in Alfin-like were down-regulated in AAS. Therefore, it was suggested that six families might play an important role in the secondary growth of A. heterophyllus stems and the response to abiotic stress.