紫草乌中生物碱成分及其对H9c2细胞毒性研究

Alkaloidal Constituents of Aconitum episcopale and Their Toxicity on H9c2 Cells

摘要:

为了解我国特有药用乌头种紫草乌(Aconitum episcopale)的生物碱类化学成分及其对大鼠心脏细胞H9c2的细胞毒性, 从紫草乌中分离出10个C₁₉-二萜生物碱，根据理化性质和波谱数据，其结构分别鉴定为滇乌碱(1)、粗茎乌头碱甲(2)、黄草乌碱丙(3)、黄草乌碱甲(4)、丽乌亭(5)、猎鹰乌头碱(6)、14-O-乙酰萨卡可尼亭(7)、萨卡可尼亭(8)、查斯曼宁(9)和展毛黄草乌碱A (10)。化合物5、6和10为首次从该植物中分离得到。化合物1~4、6~7和9~10对大鼠心脏细胞H9c2增殖的半数抑制浓度为163.8~341.6 μg/mL，表明紫草乌中的生物碱对H9c2细胞具有毒性作用。

Abstract:

To understand the alkaloidal constituents of Aconitum episcopale, an Aconitum species endemic to China, and their toxicity against rat cardiomyocyte H9c2 cells, ten diterpenoid alkaloids were extracted from its roots. Based on physical and chemical properties and spectral data, their structures were identified as yunaconitine (1), crassicauline A (2), vilmorrianine C (3), vilmorrianine A (4), forestine (5), falconeridine (6), 14-O-acetylsachaconitine (7), sachaconitine (8), chasmanine (9) and vilmotenitine A (10), were isolated and identified from A. episcopale roots. Compounds 5, 6 and 10 were isolated from this species for the first time. The median inhibition concentrations of compounds 1- 4, 6- 7 and 9- 10 on H9c2 cells ranged from 163.8 to 341.6 μg/mL, showing the alkaloids in A. episcopale had toxic effects on H9c2 cells.