蚕豆花水提取物的UPLC-ESI-QTOF质谱联用分析及抗氧化活性研究
作者:
基金项目:

福建省科技计划公益类专项(2021R1031004);福建省农业科学院科技创新团队建设项目(CXTD2021011-2);福建省“5511”协同创新工程(XTCXGC2021019)资助


UPLC-ESI-QTOF Mass Spectrometry Analysis and Antioxidant Activity of Water Extract from Vicia faba Flowers
Author:
Fund Project:

This work was supported by the Special Project for Public Welfare for Research Institute in Fujian (Grant No. 2021R1031004), the Project for Scientific and Technological Innovation Team Construction of Fujian Academy of Agricultural Sciences (Grant No. CXTD2021011-2), and the Fujian

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [54]
  • |
  • 相似文献
  • | | |
  • 文章评论
    摘要:

    为阐明蚕豆(Vicia faba)花水提取物的药效作用基础,采用超高效液相色谱-高分辨飞行时间质谱(UPLC-ESI-QTOF-MS)联用技术对其水提取物进行分析鉴定,并测定其抗氧化活性。结果表明,从蚕豆花水提取物中鉴定出178种化学成分,其中相对含量大于0.01%的成分有107种,相对含量较高的成分有野黄芩素-7-新橘皮糖苷、3-吲哚基-β-d-葡糖苷酸环己胺盐、螺环内脂E、芦丁、海藻酸丙二醇酯、山柰酚-3-α-d-半乳糖甙、4,8-二羟基喹啉-2-甲酸等。蚕豆花水提取物的主要成分以黄酮类和生物碱类为主,分别有16和17种,相对含量分别为36.53%和34.33%。清除DPPH和ABTS+自由基的IC50值分别为26.5和25.0 µg/mL。黄酮类和生物碱类是蚕豆花发挥功效的主要物质基础,蚕豆花水提取物具有较强的抗氧化活性,蚕豆花具有较高的应用价值。

    Abstract:

    In order to clarify the pharmacodynamic basis of water extract from Vicia faba flowers, the chemical constituents were analyzed and identified by ultra performance liquid chromatography electrospray ion source quadrupole time of flight mass spectrometry (UPLC-ESI-QTOF-MS), and the antioxidant activity was determined. The results showed that 178 components were identified from the water extract, and the relative contents of 107 components were more than 0.01%. The components with higher relative content included scutellarein 7-neohesperidoside, indoxyl glucuronide, spirolide E, rutin, propylene glycol alginate, kaempferol 3-α-d- galactoside, xanthurenic acid, and so on. The main components of flower water extract were flavonoids and alkaloids, with 16 and 19 kinds, and their relative contents were 36.53% and 34.33%, respectively. The IC50 of flower water extract for scavenging DPPH and ABTS+ free radical was 26.5 and 25.0 µg/mL, respectively. Therefore, flavonoids and alkaloids were the main substance basis of V. faba flower, and the water extract had strong antioxidant activity, indicating V. faba flowers had high application value.

    参考文献
    [1] WANG K H, WANG X J, MIAO Y M, et al. Breeding and cultivation of high-quality and fresh large-grain broad bean Tong-Can-Xian 8[J]. Jiangsu J Agric Sci, 2013, 41(11):113-115. doi:10.3969/j.issn.1002-1302.2013.11.043.汪凯华,王学军,缪亚梅,等.优质鲜食大粒蚕豆通蚕鲜8号的选育和栽培要点[J].江苏农业科学, 2013, 41(11):113-115. doi:10. 3969/j.issn.1002-1302.2013.11.043.
    [2] LÜ C Y, LIAO F L, CHEN H W, et al. Identification and evaluation of yield traits of 41Vicia faba germplasm resources from African areas and Hubei, China[J]. J S Agric, 2018, 49(12):2356-2363. doi:10. 3969/j.issn.2095-1191.2018.12.02.吕春雨,廖芳丽,陈宏伟,等. 41份非洲地区和我国湖北蚕豆种质资源产量性状的鉴定与评价[J].南方农业学报, 2018, 49(12):2356-2363. doi:10.3969/j.issn.2095-1191.2018.12.02.
    [3] XIA M Z, XIONG F Q. Effects of change in source-sink ratio on the anscission of broad bean flower and pod[J]. Plant Physiol Commun, 1986, 22(1):18-21. doi:10.13592/j.cnki.ppj.1986.01.005.夏明忠,熊仿秋.改变源-库比率对蚕豆花荚脱落的影响[J].植物生理学通讯, 1986, 22(1):18-21. doi:10.13592/j.cnki.ppj.1986.01. 005.
    [4] KINJO J, HATAKEYAMA M, UDAYAMA M, et al. HPLC profile analysis of oleanene-glucuronides in several edible beans[J]. Biosci Biotechnol Biochem, 1998, 62(3):429-433. doi:10.1271/bbb.62.429.
    [5] HA T J, LEE B W, PARK K H, et al. Rapid characterisation and comparison of saponin profiles in the seeds of Korean Leguminous species using ultra performance liquid chromatography with photo-diode array detector and electrospray ionisation/mass spectrometry (UPLC-PDA-ESI/MS) analysis[J]. Food Chem, 2014, 146:270-277. doi:10.1016/j.foodchem.2013.09.051.
    [6] ARISAWA M, TAKAKUWA T, HANDA K. Studies on unutilized resources:VI. The components of the flavonoids in vicia genus plants.(1). Flavonoids of Vicia faba L. and V. unijuga AL. BR.(Leguminosae)[J]. Yakugaku Zasshi, 1971, 91(5):587-589. doi:10.1248/yakushi1947. 91.5_587.
    [7] SPANOU C, VESKOUKIS A S, KERASIOTI T, et al. Flavonoid gly-colsides isolated from unique legume plant extracts as novel inhibitors of xanthine oxidase[J]. PLoS One, 2012, 7(3):e32214. doi:10.1371/journal.pone.0032214.
    [8] SPANOU C, BOUROU G, DERVISHI A, et al. Antioxidant and chemopreventive properties of polyphenolic compounds derived from Greek legume plant extracts[J]. J Agric Food Chem, 2008, 56(16):6967-6976. doi:10.1021/jf800842p.
    [9] MERGHEM R, JAY M, BRUN N, et al. Qualitative analysis and HPLC isolation and identification of procyanidins from vicia faba[J]. Phytochem Anal, 2004, 15(2):95-99. doi:10.1002/pca.731.
    [10] CATALANO G, FOSSEN T, ANDERSENØ M. Petunidin 3-O-α-rham-nopyranoside-5-O-β-glucopyranoside and other anthocyanins from flowers of Vicia villosa[J]. J Agric Food Chem, 1998, 46(11):4568-4570. doi:10.1021/jf980558e.
    [11] SINHA S K, KUMAR M, KUMAR A, et al. Antioxidant activities of different tissue extract of Faba bean (Vicia faba L.) containing phenolic compounds[J]. Legume Res, 2013, 36(6):496-504.
    [12] YAN E, LIU J L, YUAN J F, et al. Study on extraction and antioxi-dation activity of procyanidins from broad bean shell[J]. Sci Technol Food Ind, 2009, 30(2):65-67. doi:10.13386/j.issn1002-0306.2009.02. 089.阎娥,刘建利,原江锋,等.蚕豆壳中原花青素的提取及抗氧化性研究[J].食品工业科技, 2009, 30(2):65-67. doi:10.13386/j.issn 1002-0306.2009.02.089.
    [13] SIAH S D, KONCZAK I, AGBOOLA S, et al. In vitro investigations of the potential health benefits of Australian-grown faba beans (Vicia faba L.):Chemopreventative capacity and inhibitory effects on the angio-tensin-converting enzyme, α-glucosidase and lipase[J]. Br J Nutr, 2012, 108(S1):S123-S134. doi:10.1017/S0007114512000803.
    [14] XU X Y, LI A P, KANG Z M, et al. Research progress of chemical constituents and pharmacological activities of Vicia genus[J]. Chin Agric Sci Bull, 2015, 31(31):74-80.徐晓俞,李爱萍,康智明,等.野豌豆属植物化学成分及其药理活性研究进展[J].中国农学通报, 2015, 31(31):74-80.
    [15] GUO X Q, CAO J M, DAI Y W. The quality research about broadbean flower[J]. Chin J Ethnomed Ethnopharm, 2014, 23(23):6-7.郭向群,曹建民,代玉文.蚕豆花药材的质量研究[J].中国民族民间医药, 2014, 23(23):6-7.
    [16] GUO X Q, CAO J M, DAI Y W. The simultaneous content determi-nation of quercetin and kamepferol in broadbean flower by HPLC[J]. Strait Pharm J, 2014, 26(11):56-57. doi:10.3969/j.issn.1006-3765. 2014.11.020.郭向群,曹建民,代玉文. HPLC法同时测定蚕豆花中槲皮素和山奈酚的含量[J].海峡药学, 2014, 26(11):56-57. doi:10.3969/j.issn. 1006-3765.2014.11.020.
    [17] XIA M Z. Effect of environmental factors on the formation and abscission of flowers and pods[J]. J Sichuan Agric Univ, 2001, 19(4):348-351. doi:10.3969/j.issn.1000-2650.2001.04.008.夏明忠.环境因素对蚕豆花荚形成和脱落的影响[J].四川农业大学学报, 2001, 19(4):348-351. doi:10.3969/j.issn.1000-2650.2001.04. 008.
    [18] WANG Y Z, CUI K R, GONG K. Interrelation between development process of anther and ovule in Vicia faba L.[J]. Acta Bot Boreali-Occid Sin, 1986, 6(1):36-42.王耀芝,崔凯荣,宫葵.蚕豆花药和胚珠发育进程的相互关系[J].西北植物学报, 1986, 6(1):36-42.
    [19] KRAMELL R, SCHMIDT J, HERRMANN G, et al. N-(jasmonoyl) tyrosine-derived compounds from flowers of broad beans (Vicia faba)[J]. J Nat Prod, 2005, 68(9):1345-1349. doi:10.1021/np0501482.
    [20] ZHENG K B, LI A P, CAO Y Y, et al. Development of high perfor-mance liquid chromatography for determination of l-dopa from Vicia faba flower[J]. Jiangsu J Agric Sci, 2012, 28(3):688-690. doi:10. 3969/j.issn.1000-4440.2012.03.046.郑开斌,李爱萍,曹奕鸯,等.蚕豆花左旋多巴液相色谱检测方法的建立[J].江苏农业学报, 2012, 28(3):688-690. doi:10.3969/j.issn. 1000-4440.2012.03.046.
    [21] LÜ X F, ZHOU X H, WANG Y, et al. Component analysis of Dendro-bium phalaenopsis anthocyanin extract and its antioxidant activity and irritation in vitro[J]. J Trop Subtrop Bot, 2021, 29(4):374-381. doi:10.11926/jtsb.4327.吕晓帆,周新红,王莹,等.秋石斛花青素提取液成分分析及其体外抗氧化活性和刺激性研究[J].热带亚热带植物学报, 2021, 29(4):374-381. doi:10.11926/jtsb.4327.
    [22] SUN S W, LIU M J, HE J, et al. Identification and determination of seven phenolic acids in Brazilian green propolis by UPLC-ESI-QTOF-MS and HPLC[J]. Molecules, 2019, 24(9):1791. doi:10.3390/molecu les24091791.
    [23] DOS SANTOS A T L, CARNEIRO J N P, DA CRUZ R P, et al. UPLC-MS-ESI-QTOF analysis and antifungal activity of the Spondias tuberosa Arruda leaf and root hydroalcoholic extracts[J]. Antibiotics, 2019, 8(4):240. doi:10.3390/antibiotics8040240.
    [24] DONG R H, YU Q, LIAO W, et al. Composition of bound polyphenols from carrot dietary fiber and its in vivo and in vitro antioxidant activity[J]. Food Chem, 2021, 339:127879. doi:10.1016/j.foodchem.2020. 127879.
    [25] XU C, CHEN W J, YU J H, et al. Metabonomics study of selaginella tamariscina for hyperuricemia in rats using UPLC-ESI-QTOF/MS[J]. J Chin Mass Spectrom Soc, 2016, 37(5):440-445. doi:10.7538/zpxb. youxian.2016.0018.徐晨,陈维佳,于江洪,等.基于液相色谱-质谱的代谢组学方法研究卷柏治疗高尿酸血症大鼠的作用机制[J].质谱学报, 2016, 37(5):440-445. doi:10.7538/zpxb.youxian.2016.0018.
    [26] YE J, YANG M J, YANG X Y, et al. Analysis of chemical constituents in Ziziphus jujuba var. spinosa folium by UPLC-QTOF-MS[J]. Nat Prod Res Dev, 2019, 31(7):1183-1191. doi:10.16333/j.1001-6880. 2019.7.010.叶嘉,杨明建,杨香瑜,等.太行山区酸枣叶化学成分的UPLC-QTOF-MS分析[J].天然产物研究与开发, 2019, 31(7):1183-1191. doi:10.16333/j.1001-6880.2019.7.010.
    [27] BALTRUŠAITYTĖ V, VENSKUTONIS P R,ČEKSTERYTĖ V. Radical scavenging activity of different floral origin honey and beebread phenolic extracts[J]. Food Chem, 2007, 101(2):502-514. doi:10.1016/j. foodchem.2006.02.007.
    [28] GUAN Y, WANG J F, LI W, et al. Changes in major nutritional components and antioxidant activity of fermented okara[J]. Food Sci, 2016, 37(21):189-194. doi:10.7506/spkx1002-6630-201621032.管瑛,汪瑨芃,李文,等.豆渣固态发酵过程中主要营养成分及抗氧化特性变化[J].食品科学, 2016, 37(21):189-194. doi:10.7506/spkx1002-6630-201621032.
    [29] BADING-TAIKA B, AKINYEKE T, MAGANA A A, et al. Phyto-chemical characterization of Tabernanthe iboga root bark and its effects on dysfunctional metabolism and cognitive performance in high-fat-fed C57BL/6J mice[J]. JFB, 2018, 3:111-123. doi:10.31665/JFB.2018.3154.
    [30] BAO W, CAO C, LI S Q, et al. Metabonomic analysis of quercetin against the toxicity of acrylamide in rat urine[J]. Food Funct, 2017, 8(3):1204-1214. doi:10.1039/C6FO01553K.
    [31] XU X Y, LI C X, ZHENG K B, et al. Chemical constituents of water extract from Chrysanthemum morifolium flowers by UHPLC-ESI-Orbitrap MS[J]. J Trop Subtrop Bot, 2021, 29(1):96-104. doi:10. 11926/jtsb.4256.徐晓俞,李程勋,郑开斌,等.杭白菊花水提物的UHPLC-ESI-Orbitrap质谱联用分析[J].热带亚热带植物学报, 2021, 29(1):96-104. doi:10.11926/jtsb.4256.
    [32] TAN Y. Multiomics integrative analysis for discovering the mechanism of dioscin lowering uric acid in hyperuricemia mice[D]. Tianjin:Tianjin University of Traditional Chinese Medicine, 2021. doi:10. 27368/d.cnki.gtzyy.2021.000471.谈谣.多组学综合分析薯蓣皂苷对高尿酸血症小鼠的降尿酸机制[D].天津:天津中医药大学, 2021. doi:10.27368/d.cnki.gtzyy.2021. 000471.
    [33] TANG M, GAO X, GENG T, et al. Identification of chemical consti-tuents in Qiwei Tongbi oral liquid by HPLC-Q-TOF-MS/MS[J]. Chin Trad Herb Drugs, 2021, 52(8):2226-2236. doi:10.7501/j.issn.0253. 2670.2021.08.005.唐明,高霞,耿婷,等.基于HPLC-Q-TOF-MS/MS技术的七味通痹口服液化学成分分析[J].中草药, 2021, 52(8):2226-2236. doi:10.7501/j.issn.0253.2670.2021.08.005.
    [34] ZHANG Y Y. Metabolomics study of non-volatile components in the processing of fu brick tea[D]. Changsha:Hunan Agricultural University, 2019. doi:10.27136/d.cnki.ghunu.2019.000559.张贻杨.茯砖茶加工过程中非挥发性成分代谢组学研究[D].长沙:湖南农业大学, 2019. doi:10.27136/d.cnki.ghunu.2019.000559.
    [35] XU C, LOU Y, LU Q J, et al. Metabolic research of vitexin in rats[J]. Chin J Mod Appl Pharm, 2018, 35(9):1365-1369. doi:10.13748/j. cnki.issn1007-7693.2018.09.021.徐聪,楼燕,卢祺炯,等.牡荆素在大鼠体内的代谢研究[J].中国现代应用药学, 2018, 35(9):1365-1369. doi:10.13748/j.cnki. issn 1007-7693.2018.09.021.
    [36] LUO J C. Analysis of diarylheptanoids in Alpinia officinarum by UPLC/Q-TOF MS and study on anti-inflammatory activity of monomer[D]. Guangzhou:Guangdong Pharmaceutical University, 2010.罗京超.高良姜中二苯基庚烷的UPLC/Q-TOFMS分析及单体抗炎活性研究[D].广州:广东药学院, 2010.
    [37] YIN Z H, SUN C H, FANG H Z. Analysis and comparison on frag-mentation behavior of quercetin and morin by ESI-MS[J]. J Instrum Anal, 2017, 36(2):205-211. doi:10.3969/j.issn.1004-4957.2017.02. 009.尹智慧,孙长海,方洪壮.槲皮素与桑色素ESI-MS裂解行为的比较分析[J].分析测试学报, 2017, 36(2):205-211. doi:10.3969/j.issn. 1004-4957.2017.02.009.
    [38] ZHAO Y M, LIU S X, ZHANG C X, et al. Analysis on chemical constituents from glycyrrhizae radix et rhizoma by HPLC-Q-TOF-MS[J]. Chin Trad Herb Drugs, 2016, 47(12):2061-2068. doi:10.7501/j.issn.0253-2670.2016.12.007.赵艳敏,刘素香,张晨曦,等.基于HPLC-Q-TOF-MS技术的甘草化学成分分析[J].中草药, 2016, 47(12):2061-2068. doi:10.7501/j. issn.0253-2670.2016.12.007.
    [39] XU D C, LIU J, LI X J, et al. Study on absorbed components of Xiebai powder in rat blood[J]. China Pharm, 2022, 33(1):38-45. doi:10. 6039/j.issn.1001-0408.2022.01.07.徐东川,刘瑾,李晓晶,等.泻白散大鼠体内入血成分研究[J].中国药房, 2022, 33(1):38-45. doi:10.6039/j.issn.1001-0408.2022.01.07.
    [40] ZHAO X Y, FU X F, LI J, et al. Determination of salicin, a charac-teristic component of poplar tree gum by HPLC-LTQ-Orbitrap high resolution mass spectrometry[J]. J Instrum Anal, 2016, 35(3):342-346. doi:10.3969/j.issn.1004-4957.2016.03.015.赵晓亚,付晓芳,李晶,等.高效液相色谱-线性离子阱-静电场轨道阱高分辨质谱测定杨树胶的指标性成分水杨苷[J].分析测试学报, 2016, 35(3):342-346. doi:10.3969/j.issn.1004-4957.2016.03.015.
    [41] FANG G Z, ZHANG L F, ZHANG L, et al. Use of two-dimensional nuclear magnetic resonance technique for identifying the geometric conformation of farnesylacetone[J]. Chin Rem Clin, 2019, 19(9):1397-1400. doi:10.11655/zgywylc2019.09.002.房桂珍,张丽芳,张莉,等.二维核磁共振技术确定法尼基丙酮的几何构型[J].中国药物与临床, 2019, 19(9):1397-1400. doi:10. 11655/zgywylc2019.09.002.
    [42] LI Y Z, WANG J D, YUE C C, et al. Study on the effect mechanism of scutellarin in the occurrence and development of colon cancer[J]. China Mod Med, 2021, 28(20):12-16. doi:10.3969/j.issn.1674-4721. 2021.20.005.李远志,王钧冬,岳朝驰,等.野黄芩素在结肠癌发生发展中的作用机制研究[J].中国当代医药, 2021, 28(20):12-16. doi:10.3969/j. issn.1674-4721.2021.20.005.
    [43] YANG Y, JIANG C H, JIN Q M, et al. Effect of scutellarein on acute pharyngitis in rats[J]. J China Pharm Univ, 2019, 50(5):600-605. doi:10.11665/j.issn.1000-5048.20190514.杨阳,蒋翠花,金乔梅,等.野黄芩素对大鼠急性咽炎的治疗作用[J].中国药科大学学报, 2019, 50(5):600-605. doi:10.11665/j.issn. 1000-5048.20190514.
    [44] ZHONG W L, XIONG Y, WANG X W, et al. Anti-tumor effect and mechanism of kaempferol:A review[J]. Chin J Exp Trad Med Formul, 2021, 27(20):219-226. doi:10.13422/j.cnki.syfjx.20212025.钟文良,熊雨,王贤文,等.山柰酚抗肿瘤效应与机制研究进展[J].中国实验方剂学杂志, 2021, 27(20):219-226. doi:10.13422/j.cnki. syfjx.20212025.
    [45] MAO Y J, FENG Y L, WANG M J, et al. Research progress on rutin derivatives[J]. China J Chin Mat Med, 2021, 46(18):4654-4665. doi:10.19540/j.cnki.cjcmm.20210429.602.毛雅君,冯亚莉,王梦娇,等.芦丁衍生物的研究进展[J].中国中药杂志, 2021, 46(18):4654-4665. doi:10.19540/j.cnki.cjcmm.20210429.602.
    [46] YAO L, XU L X, XUE J H, et al. Antibacterial activity and metabolits of an acremonium fungus[J]. J Trop Subtrop Bot, 2012, 20(2):192-196. doi:10.3969/j.issn.1005-3395.2012.02.014.姚磊,徐良雄,薛璟花,等.枝顶孢属真菌的抑菌活性及其代谢产物研究[J].热带亚热带植物学报, 2012, 20(2):192-196. doi:10. 3969/j.issn.1005-3395.2012.02.014.
    [47] SONG S L, LI B X, HAN Y F. Inhibitory effect of sphingosine on human colon HT-29 cells[J]. Chin J Publ Health, 2004, 20(7):775-776. doi:10.3321/j.issn:1001-0580.2004.07.003.宋士利,李百祥,韩云峰.鞘氨醇对人结肠癌细胞的抑制作用[J].中国公共卫生, 2004, 20(7):775-776. doi:10.3321/j.issn:1001-0580. 2004.07.003.
    [48] LU R. Preparation and characterization of glucuronic acid and its lactone[D]. Xian:Shaanxi University of Science& Technology, 2018.卢瑞.葡萄糖醛酸及其内酯的制备与表征[D].西安:陕西科技大学, 2018.
    [49] GU Y C, QIAN L Q, LI B X, et al. Synthesis of linalool oxide[J]. Flav Frag Cosm, 2013(S1):28-31. doi:10.3969/j.issn.1000-4475.2013.z1. 009.谷运璀,钱莉群,李步详,等.芳樟醇氧化物的合成[J].香料香精化妆品, 2013(S1):28-31. doi:10.3969/j.issn.1000-4475.2013.z1.009.
    [50] LI S P, HUANG B X, LIU C Y, et al. Rapid determination of vitamin A in food by pyrolysis derivatization gas chromatography triple qua-drupole mass spectrometry[J]. J Food Saf Qual, 2021, 12(4):1281-1290. doi:10.19812/j.cnki.jfsq11-5956/ts.2021.04.004.李少鹏,黄濒漩,刘聪熠,等.热裂解衍生气相色谱-三重四极杆质谱法快速测定食品中维生素A[J].食品安全质量检测学报, 2021, 12(4):1281-1290. doi:10.19812/j.cnki.jfsq11-5956/ts.2021.04.004.
    [51] WANG Z Z, YANG Y H, ZHANG H X, et al. Volatile and aroma components of Gynostemma pentaphyllum(Thunb.) Makino tea from five different origins[J]. Nat Prod Res Dev, 2020, 32(10):1717-1729. doi:10.16333/j.1001-6880.2020.10.013.王忠泽,杨宇涵,张红霞,等.五个不同产地绞股蓝茶挥发物及香气成分研究[J].天然产物研究与开发, 2020, 32(10):1717-1729. doi:10.16333/j.1001-6880.2020.10.013.
    [52] DENG S R, PAN Y Z. Research on main chemical constituents and pharmacological effects of cinnamon[J]. Mod J Integr Trad Chin West Med, 2018, 27(4):448-451. doi:10.3969/j.issn.1008-8849.2018.04.034.邓淑蓉,潘宇政.肉桂主要化学成分及药理作用研究概况[J].现代中西医结合杂志, 2018, 27(4):448-451. doi:10.3969/j.issn.1008-8849.2018.04.034.
    [53] YANG H, BAI X F, YANG L Y, et al. A methodological study of the determination of α-curcumene in Sri Lanka Turmeric[J]. J Yunnan Minzu Univ (Nat Sci), 2020, 29(3):190-194. doi:10.3969/j.issn.1672-8513.2020.03.002.杨瀚,白雪飞,杨丽英,等.斯里兰卡姜黄中α-姜黄烯含量测定的方法学研究[J].云南民族大学学报(自然科学版), 2020, 29(3):190-194. doi:10.3969/j.issn.1672-8513.2020.03.002.
    [54] CHEN X Q, LI S P, ZHAO J. Ganoderma triterpenoids from aqueous extract of Ganoderma lucidum[J]. China J Chin Mat Med, 2017, 42(10):1908-1915. doi:10.19540/j.cnki.cjcmm.20170412.001.陈显强,李绍平,赵静.赤芝水提取物中的三萜类成分[J].中国中药杂志, 2017, 42(10):1908-1915. doi:10.19540/j.cnki.cjcmm.20170412.001.
    相似文献
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

李程勋,徐晓俞,李爱萍,郑开斌.蚕豆花水提取物的UPLC-ESI-QTOF质谱联用分析及抗氧化活性研究[J].热带亚热带植物学报,2022,30(5):663~675

复制
分享
文章指标
  • 点击次数:193
  • 下载次数: 358
  • HTML阅读次数: 447
  • 引用次数: 0
历史
  • 收稿日期:2021-12-17
  • 最后修改日期:2022-05-06
  • 录用日期:2022-05-13
  • 在线发布日期: 2022-09-26
文章二维码