蚕豆花水提取物的UPLC-ESI-QTOF质谱联用分析及抗氧化活性研究

UPLC-ESI-QTOF Mass Spectrometry Analysis and Antioxidant Activity of Water Extract from Vicia faba Flowers

摘要:

为阐明蚕豆(Vicia faba)花水提取物的药效作用基础，采用超高效液相色谱-高分辨飞行时间质谱(UPLC-ESI-QTOF-MS)联用技术对其水提取物进行分析鉴定，并测定其抗氧化活性。结果表明，从蚕豆花水提取物中鉴定出178种化学成分，其中相对含量大于0.01%的成分有107种，相对含量较高的成分有野黄芩素-7-新橘皮糖苷、3-吲哚基-β-d-葡糖苷酸环己胺盐、螺环内脂E、芦丁、海藻酸丙二醇酯、山柰酚-3-α-d-半乳糖甙、4,8-二羟基喹啉-2-甲酸等。蚕豆花水提取物的主要成分以黄酮类和生物碱类为主，分别有16和17种，相对含量分别为36.53%和34.33%。清除DPPH和ABTS⁺自由基的IC₅₀值分别为26.5和25.0 µg/mL。黄酮类和生物碱类是蚕豆花发挥功效的主要物质基础，蚕豆花水提取物具有较强的抗氧化活性，蚕豆花具有较高的应用价值。

Abstract:

In order to clarify the pharmacodynamic basis of water extract from Vicia faba flowers, the chemical constituents were analyzed and identified by ultra performance liquid chromatography electrospray ion source quadrupole time of flight mass spectrometry (UPLC-ESI-QTOF-MS), and the antioxidant activity was determined. The results showed that 178 components were identified from the water extract, and the relative contents of 107 components were more than 0.01%. The components with higher relative content included scutellarein 7-neohesperidoside, indoxyl glucuronide, spirolide E, rutin, propylene glycol alginate, kaempferol 3-α-d- galactoside, xanthurenic acid, and so on. The main components of flower water extract were flavonoids and alkaloids, with 16 and 19 kinds, and their relative contents were 36.53% and 34.33%, respectively. The IC₅₀ of flower water extract for scavenging DPPH and ABTS⁺ free radical was 26.5 and 25.0 µg/mL, respectively. Therefore, flavonoids and alkaloids were the main substance basis of V. faba flower, and the water extract had strong antioxidant activity, indicating V. faba flowers had high application value.