铜藻多糖对H2O2诱导的HaCaT细胞氧化应激的保护作用

Protective Effect of Polysaccharide from Sargassum horneri on H2O2-induced Oxidative Stress in HaCaT Cells

摘要:

为探究铜藻多糖（Sargassum horneri polysaccharides，SHP）对H₂O₂诱导的人角质形成细胞（HaCaT）氧化应激损伤的保护作用，测定了SHP对总抗氧化能力（T-AOC）、DPPH自由基、羟自由基（·OH）和超氧阴离子自由基（O₂^·）的清除作用，以评价SHP的体外抗氧化能力，并建立H₂O₂诱导HaCaT细胞氧化损伤模型；通过测定细胞存活率、细胞活性氧以及酶活，评价SHP对HaCaT细胞氧化损伤的保护作用。结果表明，当SHP为1 mg/mL时，DPPH的清除率为68%、·OH清除能力65.48 U/mL；在SHP为3 mg/mL时，O₂^·清除能力为84.86 U/mL，T-AOC为33.55。SHP能显著提高H₂O₂诱导氧化损伤的HaCaT细胞活力，其中经100 μg/mL SHP处理后，HaCaT细胞存活率由56.85%提高到80.57%，并且显著降低细胞内ROS水平，提高细胞内SOD活力，减少细胞内MDA的含量（P<0.05）。因此，SHP对H₂O₂诱导的HaCaT细胞氧化应激损伤具有保护作用，SHP可作为一种天然的抗氧化剂在化妆品以及药妆品领域具有广阔的应用前景。

Abstract:

To investigate the protective effect of Sargassum horneri polysaccharides (SHP) on H₂O₂-induced oxidative stress injury of human keratinocytes (HaCaT), the total antioxidant capacity (T-AOC), DPPH free radical, hydroxyl free radical (·OH) and superoxide anion free radical (O₂^·) were determined, and the antioxidant capacity of SHP in vitro and established the oxidative damage model of HaCaT cells induced by H₂O₂was evaluated. The protective effect of SHP on oxidative damage of HaCaT cells was evaluated by measuring cell survival rate, reactive oxygen species and enzyme activity. The results showed that the clearance rate of DPPH was 68% and the scavenging capacity of ·OH was 65.48 U/mL treated with 1 mg/mL SHP. The clearance capacity of O₂^· was 84.86 U/mL and T-AOC was 33.55 treated with 3 mg/mL SHP. SHP could significantly improve the viability of HaCaT cells by H₂O₂-induced oxidative damage. After, The survival rate of HaCaT cells increased from 56.85% to 80.57%, as well as the intracellular SOD activity treated with 100 μg/mL SHP, while the contents of ROS and MDA significantly reduced (P<0.05). Therefore, SHP had a protective effect on H₂O₂-induced oxidative stress injury of HaCaT cells, suggesting that SHP, a natural antioxidant, could be widely applied to cosmetics and cosmeceuticals.